Experiment set2IT022 for Pseudomonas fluorescens FW300-N2E2

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L-Histidine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-Histidine (20 mM), pH=7
Culturing: pseudo6_N2E2_ML5, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 3/9/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 21 genes in this experiment

For carbon source L-Histidine in Pseudomonas fluorescens FW300-N2E2

For carbon source L-Histidine across organisms

SEED Subsystems

Subsystem #Specific
Histidine Degradation 5
Coenzyme PQQ synthesis 2
Pyrroloquinoline Quinone biosynthesis 2
ABC transporter alkylphosphonate (TC 3.A.1.9.1) 1
Cobalt-zinc-cadmium resistance 1
Glycolysis and Gluconeogenesis 1
Glycolysis and Gluconeogenesis, including Archaeal enzymes 1
Purine Utilization 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Ribosome biogenesis bacterial 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-histidine degradation II 5 5 5
L-histidine degradation I 4 4 4
L-histidine degradation III 6 4 3
L-histidine degradation VI 8 7 3
pyrroloquinoline quinone biosynthesis 7 4 2
glycolysis V (Pyrococcus) 10 7 1
glycolysis II (from fructose 6-phosphate) 11 9 1
gluconeogenesis I 13 11 1
glycolysis I (from glucose 6-phosphate) 13 10 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 14 1
superpathway of hexitol degradation (bacteria) 18 12 1
gluconeogenesis II (Methanobacterium thermoautotrophicum) 18 9 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of anaerobic sucrose degradation 19 14 1
superpathway of N-acetylneuraminate degradation 22 13 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 21 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1