Experiment set2IT021 for Methanococcus maripaludis S2

200 most detrimental genes:

gene	name	fitness	t score	description
MMP_RS02145		+2.3	14.2	winged helix-turn-helix transcriptional regulator
MMP_RS01960		+2.2	2.0	hypothetical protein
MMP_RS01900		+2.1	17.1	hypothetical protein
MMP_RS01925		+2.1	13.5	DUF1616 domain-containing protein
MMP_RS01265		+2.0	3.6	hypothetical protein
MMP_RS01905		+1.9	15.6	glycosyltransferase
MMP_RS01885		+1.6	9.3	Gfo/Idh/MocA family oxidoreductase
MMP_RS01880		+1.5	8.9	DegT/DnrJ/EryC1/StrS family aminotransferase
MMP_RS01970		+1.4	10.9	TIGR04279 domain-containing protein
MMP_RS01915		+1.4	11.0	DUF354 domain-containing protein
MMP_RS01875		+1.4	9.3	N-acetyltransferase
MMP_RS01890		+1.3	5.6	nucleotide sugar dehydrogenase
MMP_RS01910		+1.1	7.6	UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
MMP_RS01955		+1.1	4.2	hypothetical protein
MMP_RS07075		+1.1	4.6	phosphomannomutase/phosphoglucomutase
MMP_RS01920		+1.1	9.0	glycosyltransferase family 2 protein
MMP_RS07860		+1.1	1.0	hypothetical protein
MMP_RS06840		+1.1	1.5	DNA-directed RNA polymerase subunit N
MMP_RS06400		+1.1	2.3	4Fe-4S binding protein
MMP_RS06670		+1.0	4.8	glucose-6-phosphate isomerase
MMP_RS06310		+1.0	3.7	NAD(P)/FAD-dependent oxidoreductase
MMP_RS04870		+1.0	5.5	TIGR02253 family HAD-type hydrolase
MMP_RS03300		+1.0	1.2	TOBE domain-containing protein
MMP_RS07545		+0.9	1.5	HTH domain-containing protein
MMP_RS08745		+0.9	5.3	CDP-2 3-bis-(O-geranylgeranyl)-sn-glycerol synthase
MMP_RS00675		+0.9	1.2	nitroreductase family protein
MMP_RS06460		+0.9	1.6	pyrimidine dimer DNA glycosylase/endonuclease V
MMP_RS01380		+0.9	3.8	PHP domain-containing protein
MMP_RS02175		+0.8	1.0	DUF1611 domain-containing protein
MMP_RS08300		+0.8	0.8	metal-sulfur cluster assembly factor
MMP_RS06110		+0.8	3.0	ATP-dependent protease LonB
MMP_RS03080		+0.8	2.2	universal stress protein
MMP_RS05835		+0.8	1.2	secondary thiamine-phosphate synthase enzyme YjbQ
MMP_RS08595		+0.8	1.0	flagellar protein C
MMP_RS07635		+0.7	4.1	MogA/MoaB family molybdenum cofactor biosynthesis protein
MMP_RS00060		+0.7	2.2	3-dehydroquinate synthase II
MMP_RS04615		+0.7	1.5	PDGLE domain-containing protein
MMP_RS00720		+0.7	3.8	metal-dependent hydrolase
MMP_RS03450		+0.7	1.4	glycine synthesis protein GlyXS (from data)
MMP_RS05550		+0.7	0.8	cation:proton antiporter
MMP_RS07345		+0.7	3.1	glycine synthesis protein GlyXL (from data)
MMP_RS00520		+0.7	1.3	RNA polymerase Rpb4 family protein
MMP_RS00130		+0.7	1.0	nickel-responsive transcriptional regulator NikR
MMP_RS00695		+0.7	1.0	biotin--[acetyl-CoA-carboxylase] ligase
MMP_RS01940		+0.6	2.0	MoxR family ATPase
MMP_RS06870		+0.6	2.6	DedA family protein
MMP_RS08195		+0.6	4.1	hypothetical protein
MMP_RS02355		+0.6	1.2	30S ribosomal protein S27ae
MMP_RS08650		+0.6	2.3	glutamine--fructose-6-phosphate transaminase (isomerizing)
MMP_RS06175		+0.6	3.6	winged helix-turn-helix transcriptional regulator
MMP_RS08615		+0.6	1.6	flagellar protein G
MMP_RS07630		+0.6	1.3	ATP-binding cassette domain-containing protein
MMP_RS05640		+0.6	3.0	UTP--glucose-1-phosphate uridylyltransferase GalU
MMP_RS00515		+0.6	0.9	DUF655 domain-containing protein
MMP_RS06905		+0.6	1.8	hypothetical protein
MMP_RS03465		+0.6	1.0	adenine phosphoribosyltransferase
MMP_RS02675		+0.6	2.3	hypothetical protein
MMP_RS03960		+0.5	0.9	hypothetical protein
MMP_RS08320		+0.5	2.5	molybdopterin molybdotransferase MoeA
MMP_RS07765		+0.5	3.0	sodium:alanine symporter family protein
MMP_RS08215		+0.5	2.0	DUF483 domain-containing protein
MMP_RS03475		+0.5	0.5	hypothetical protein
MMP_RS02605		+0.5	0.7	MotA/TolQ/ExbB proton channel family protein
MMP_RS04720		+0.5	1.7	DedA family protein
MMP_RS05515		+0.5	2.7	cyclic pyranopterin monophosphate synthase MoaC
MMP_RS05975		+0.5	1.1	hypothetical protein
MMP_RS05850		+0.5	1.5	Ldh family oxidoreductase
MMP_RS08720		+0.5	1.2	4Fe-4S binding protein
MMP_RS05315		+0.5	1.1	hypothetical protein
MMP_RS08460		+0.5	1.6	proteasome-activating nucleotidase
MMP_RS03020		+0.5	2.1	peptidylprolyl isomerase
MMP_RS06540		+0.5	1.8	3-methyl-2-oxobutanoate dehydrogenase subunit VorB
MMP_RS01975		+0.5	3.9	hypothetical protein
MMP_RS07560		+0.5	2.3	prefoldin subunit alpha
MMP_RS07840		+0.5	1.1	ribonuclease III
MMP_RS04020		+0.5	2.0	hypothetical protein
MMP_RS01500		+0.5	1.3	CBS domain-containing protein
MMP_RS07350		+0.4	1.4	preprotein translocase subunit Sec61beta
MMP_RS08980		+0.4	2.4	hypothetical protein
MMP_RS03640		+0.4	2.0	archaeal proteasome endopeptidase complex subunit beta
MMP_RS04695		+0.4	1.9	selenide water dikinase SelD
MMP_RS06295		+0.4	2.4	methanogenesis marker 14 protein
MMP_RS04710		+0.4	0.9	hypothetical protein
MMP_RS06155		+0.4	1.0	DUF4186 domain-containing protein
MMP_RS06355		+0.4	1.3	molybdenum cofactor biosynthesis protein MoaE
MMP_RS01650		+0.4	0.6	sulfurtransferase TusA family protein
MMP_RS08210		+0.4	1.0	phosphatidylglycerophosphatase A
MMP_RS06610		+0.4	1.2	DUF2334 domain-containing protein
MMP_RS05675		+0.4	0.7	phosphate ABC transporter ATP-binding protein PstB
MMP_RS07850		+0.4	1.6	prephenate dehydratase
MMP_RS02300		+0.4	2.2	NAD(P)H-dependent oxidoreductase
MMP_RS02370		+0.4	1.2	NifB/NifX family molybdenum-iron cluster-binding protein
MMP_RS08670		+0.4	2.1	metallophosphoesterase
MMP_RS02045		+0.4	1.0	tRNA-Met
MMP_RS04815		+0.4	1.9	chemotaxis protein CheD
MMP_RS02745		+0.4	0.7	hypothetical protein
MMP_RS01600		+0.4	1.3	50S ribosomal protein L15e
MMP_RS01175		+0.4	2.0	triphosphoribosyl-dephospho-CoA synthase
MMP_RS08395		+0.4	1.7	DsrE family protein
MMP_RS03520		+0.4	0.6	YwbE family protein
MMP_RS01935		+0.4	3.1	DUF58 domain-containing protein
MMP_RS03540		+0.4	1.5	DUF2178 domain-containing protein
MMP_RS02955		+0.4	1.0	DJ-1/PfpI family protein
MMP_RS01800		+0.4	1.7	TIGR00303 family protein
MMP_RS05310		+0.4	2.4	hypothetical protein
MMP_RS07735		+0.4	0.5	2-ketoisovalerate ferredoxin oxidoreductase subunit alpha
MMP_RS06765		+0.4	1.8	flap endonuclease-1
MMP_RS06590		+0.4	1.6	GNAT family N-acetyltransferase
MMP_RS04035		+0.4	0.9	hypothetical protein
MMP_RS01945		+0.3	2.2	DUF4350 domain-containing protein
MMP_RS04430		+0.3	2.2	DUF1847 domain-containing protein
MMP_RS09020		+0.3	0.6	hypothetical protein
MMP_RS03890		+0.3	1.3	DUF2540 domain-containing protein
MMP_RS02245		+0.3	1.5	hypothetical protein
MMP_RS07575		+0.3	1.2	RNA ligase partner protein
MMP_RS01950		+0.3	2.7	hypothetical protein
MMP_RS03025		+0.3	1.6	molybdenum cofactor guanylyltransferase
MMP_RS03495		+0.3	1.8	Na/Pi cotransporter family protein
MMP_RS07555		+0.3	0.3	monovalent cation/H+ antiporter subunit E
MMP_RS03015		+0.3	1.7	GTP 3' 8-cyclase MoaA
MMP_RS01660		+0.3	1.5	hypothetical protein
MMP_RS03535		+0.3	0.7	helix-turn-helix transcriptional regulator
MMP_RS00830		+0.3	1.2	purine phosphoribosyltransferase family protein
MMP_RS01930		+0.3	2.7	hypothetical protein
MMP_RS01150		+0.3	1.2	aldo/keto reductase
MMP_RS06495		+0.3	0.8	YkgJ family cysteine cluster protein
MMP_RS07900		+0.3	1.1	YeeE/YedE family protein
MMP_RS00725		+0.3	1.8	5 10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC
MMP_RS06285		+0.3	0.8	precorrin-6Y C5 15-methyltransferase (decarboxylating) subunit CbiT
MMP_RS07625		+0.3	1.1	cobalt ECF transporter T component CbiQ
MMP_RS02235		+0.3	2.1	CBS domain-containing protein
MMP_RS06075		+0.3	1.6	class I SAM-dependent methyltransferase
MMP_RS04875		+0.3	1.6	energy-converting hydrogenase B subunit P
MMP_RS05845		+0.3	0.6	tRNA-intron lyase
MMP_RS01665		+0.3	1.1	DUF2111 domain-containing protein
MMP_RS06915		+0.3	1.5	metallophosphoesterase
MMP_RS04635		+0.3	1.6	GNAT family N-acetyltransferase
MMP_RS04030		+0.3	1.0	hypothetical protein
MMP_RS05910		+0.3	1.0	cell division ATPase MinD
MMP_RS09055		+0.3	0.5	CAP domain-containing protein
MMP_RS02885		+0.3	2.3	molybdopterin biosynthesis protein
MMP_RS05090		+0.3	1.3	CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta
MMP_RS07955		+0.3	1.5	FumA C-terminus/TtdB family hydratase beta subunit
MMP_RS06970		+0.3	1.2	ribosomal protein S18-alanine N-acetyltransferase
MMP_RS08230		+0.3	1.1	50S ribosomal protein L11 methyltransferase
MMP_RS04285		+0.3	1.5	Ni/Fe hydrogenase subunit alpha
MMP_RS08350		+0.3	0.4	hypothetical protein
MMP_RS07770		+0.3	1.6	alanine racemase
MMP_RS06225		+0.3	1.5	DNA double-strand break repair nuclease NurA
MMP_RS04260		+0.3	1.4	coenzyme F420 hydrogenase subunit gamma
MMP_RS00905		+0.3	1.0	phenylacetate--CoA ligase
MMP_RS02960		+0.3	0.9	class I SAM-dependent methyltransferase family protein
MMP_RS06975		+0.3	0.8	sulfide-dependent adenosine diphosphate thiazole synthase
MMP_RS01895		+0.3	2.0	flippase
MMP_RS01830		+0.3	0.6	tRNA-Val
MMP_RS08310		+0.3	0.8	TMEM165/GDT1 family protein
MMP_RS07200		+0.3	1.4	[5-(aminomethyl)furan-3-yl]methyl phosphate kinase
MMP_RS04150		+0.3	1.8	MFS transporter
MMP_RS06525		+0.3	0.5	YciI family protein
MMP_RS06940		+0.3	1.1	polyprenyl diphosphate synthase
MMP_RS03120		+0.3	0.7	hypothetical protein
MMP_RS05440		+0.3	1.3	winged helix-turn-helix transcriptional regulator
MMP_RS05865		+0.3	1.1	hypothetical protein
MMP_RS05170		+0.3	1.3	lysine exporter LysO family protein
MMP_RS04420		+0.3	1.1	LysE family translocator
MMP_RS07525		+0.3	1.5	4Fe-4S binding protein
MMP_RS08600		+0.3	0.7	flagellar protein D
MMP_RS08635		+0.3	0.5	UbiA family prenyltransferase
MMP_RS05825		+0.2	0.7	peptidyl-prolyl cis-trans isomerase
MMP_RS00080		+0.2	1.1	RecB-family nuclease
MMP_RS02475		+0.2	0.7	hypothetical protein
MMP_RS05970		+0.2	0.7	class II SORL domain-containing protein
MMP_RS01020		+0.2	0.4	3 4-dihydroxy-2-butanone-4-phosphate synthase
MMP_RS03435		+0.2	0.6	ketol-acid reductoisomerase
MMP_RS03845		+0.2	1.5	DNA adenine methylase
MMP_RS01805		+0.2	0.9	NUDIX hydrolase
MMP_RS08570		+0.2	1.5	coenzyme F420-0:L-glutamate ligase
MMP_RS04315		+0.2	1.6	corrinoid protein
MMP_RS04895		+0.2	2.0	AI-2E family transporter
MMP_RS01325		+0.2	0.5	dihydroneopterin aldolase family protein
MMP_RS05425		+0.2	1.1	monovalent cation/H+ antiporter complex subunit F
MMP_RS00335		+0.2	0.9	5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase CofH
MMP_RS04605		+0.2	0.6	cobalt ECF transporter T component CbiQ
MMP_RS00895		+0.2	0.8	hypothetical protein
MMP_RS00565		+0.2	0.8	CBS domain-containing protein
MMP_RS01445		+0.2	0.9	hypothetical protein
MMP_RS04125		+0.2	1.0	TetR/AcrR family transcriptional regulator
MMP_RS02765		+0.2	1.0	ABC transporter ATP-binding protein
MMP_RS04215		+0.2	1.1	nickel pincer cofactor biosynthesis protein LarB
MMP_RS01250		+0.2	0.7	tRNA (guanine(26)-N(2))-dimethyltransferase
MMP_RS03865		+0.2	1.6	aspartate/glutamate racemase family protein
MMP_RS05820		+0.2	1.0	RNA-binding transcriptional accessory protein
MMP_RS04060		+0.2	1.3	hypothetical protein
MMP_RS00535		+0.2	1.0	TIGR00375 family protein
MMP_RS01570		+0.2	0.5	hypothetical protein
MMP_RS05830		+0.2	0.9	peptidyl-prolyl cis-trans isomerase
MMP_RS04945		+0.2	0.9	hypothetical protein
MMP_RS04780		+0.2	0.8	ribonuclease P
MMP_RS07705		+0.2	1.1	transcriptional regulator
MMP_RS02410		+0.2	0.9	ABC transporter substrate-binding protein

Specific Phenotypes

None in this experiment

For Methanococcus maripaludis S2 in freezer experiments