Experiment set2IT019 for Desulfovibrio vulgaris Miyazaki F
MoYLS4 with Polymyxin B sulfate 0.125 mg/ml
Group: stressMedia: MoYLS4 + Polymyxin B sulfate (0.125 mg/ml), pH=7.2
Culturing: Miya_ML3, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Jen on 11/23/2014
Media components: 1 g/L Yeast Extract, 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate, Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)
Specific Phenotypes
For 5 genes in this experiment
For stress Polymyxin B sulfate in Desulfovibrio vulgaris Miyazaki F
For stress Polymyxin B sulfate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Formate hydrogenase | 2 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| formate oxidation to CO2 | 1 | 1 | 1 |
| oxalate degradation VI | 4 | 1 | 1 |
| oxalate degradation III | 5 | 1 | 1 |
| superpathway of C1 compounds oxidation to CO2 | 12 | 2 | 1 |
| purine nucleobases degradation I (anaerobic) | 15 | 5 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 1 |