Experiment set2IT012 for Caulobacter crescentus NA1000
Sodium nitrate nitrogen source
Group: nitrogen sourceMedia: M2_noNitrogen + Sodium nitrate (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.3 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 20 mM D-Glucose, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1527 D2
Specific Phenotypes
For 8 genes in this experiment
For nitrogen source Sodium nitrate in Caulobacter crescentus NA1000
For nitrogen source Sodium nitrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Nitrate and nitrite ammonification | 4 |
| Molybdenum cofactor biosynthesis | 3 |
| Transport of Molybdenum | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| alkylnitronates degradation | 2 | 2 | 1 |
| nitrate reduction V (assimilatory) | 2 | 1 | 1 |
| molybdopterin biosynthesis | 6 | 3 | 1 |