Experiment set2IT011 for Azospirillum brasilense Sp245
L-Proline carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + L-Proline (20 mM), pH=7
Culturing: AzoBra_ML2a, 24 deep-well microplate; Multitron, Aerobic, at 30 (C), shaken=750 rpm
By: Mark on 3/5/2015
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 5 genes in this experiment
For carbon source L-Proline in Azospirillum brasilense Sp245
For carbon source L-Proline across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| ABC transporter branched-chain amino acid (TC 3.A.1.4.1) | 1 |
| Arginine and Ornithine Degradation | 1 |
| Proline, 4-hydroxyproline uptake and utilization | 1 |
| Respiratory dehydrogenases 1 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-proline degradation I | 3 | 3 | 2 |
| proline to cytochrome bo oxidase electron transfer | 2 | 2 | 1 |
| L-arginine degradation I (arginase pathway) | 3 | 3 | 1 |
| ethene biosynthesis II (microbes) | 4 | 1 | 1 |
| (5R)-carbapenem carboxylate biosynthesis | 6 | 1 | 1 |
| L-Nδ-acetylornithine biosynthesis | 7 | 6 | 1 |
| L-citrulline biosynthesis | 8 | 8 | 1 |
| superpathway of L-citrulline metabolism | 12 | 10 | 1 |