Experiment set2IT010 for Synechococcus elongatus PCC 7942
Light Dark second sampling rep2
Group: light-darkMedia: BG11, pH=7.5
Culturing: SynE_ML6, phenometrics bioreactor, Aerobic, at 30 (C), shaken=150 rpm
Growth: about 6.0 generations
By: Ben Rubin on 9/17/2014
Media components: 1.5 g/L Sodium nitrate, 0.04 g/L Potassium phosphate dibasic, 0.075 g/L Magnesium Sulfate Heptahydrate, 0.036 g/L Calcium chloride dihydrate, 0.006 g/L Citric Acid, 0.006 g/L Ferric ammonium citrate, 0.001 g/L EDTA (disodium salt), 0.02 g/L Sodium carbonate, Trace metal mix A5 (0.00286 g/L Boric Acid, 0.00181 g/L Manganese (II) chloride tetrahydrate, 0.000222 g/L Zinc sulfate heptahydrate, 0.00039 g/L Sodium Molybdate Dihydrate, 7.9e-05 g/L Copper (II) sulfate pentahydrate, 0.0494 mg/L Cobalt(II) nitrate hexahydrate)
Specific Phenotypes
For 3 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Alginate metabolism | 1 |
| Entner-Doudoroff Pathway | 1 |
| Mannose Metabolism | 1 |
| Potassium homeostasis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| GDP-mannose biosynthesis | 4 | 4 | 2 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 10 | 2 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 10 | 2 |
| β-(1,4)-mannan degradation | 7 | 2 | 1 |