Experiment set2IT008 for Pseudomonas fluorescens FW300-N2E3
LB with Thallium(I) acetate 0.008 mg/ml
Group: stressMedia: LB + Thallium(I) acetate (0.008 mg/ml)
Culturing: pseudo3_N2E3_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jayashree on 4/28/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 892 E1,E2
Specific Phenotypes
For 4 genes in this experiment
For stress Thallium(I) acetate in Pseudomonas fluorescens FW300-N2E3
For stress Thallium(I) acetate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Choline and Betaine Uptake and Betaine Biosynthesis | 2 |
| Biogenesis of cytochrome c oxidases | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycine betaine biosynthesis I (Gram-negative bacteria) | 2 | 2 | 1 |
| choline degradation I | 2 | 2 | 1 |
| glycine betaine biosynthesis II (Gram-positive bacteria) | 2 | 2 | 1 |
| choline-O-sulfate degradation | 3 | 3 | 1 |
| NADPH to cytochrome c oxidase via plastocyanin (thylakoid membrane) | 4 | 1 | 1 |
| photosynthesis light reactions | 4 | 1 | 1 |
| superpathway of photosynthetic hydrogen production | 6 | 2 | 1 |
| oxygenic photosynthesis | 17 | 11 | 1 |
| ethene biosynthesis V (engineered) | 25 | 19 | 1 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 20 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |