Experiment set2IT008 for Caulobacter crescentus NA1000

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Inosine nitrogen source

Group: nitrogen source
Media: M2_noNitrogen + Inosine (5 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.5 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 20 mM D-Glucose, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1527 C2

Specific Phenotypes

For 9 genes in this experiment

For nitrogen source Inosine in Caulobacter crescentus NA1000

For nitrogen source Inosine across organisms

SEED Subsystems

Subsystem #Specific
Molybdenum cofactor biosynthesis 3
Purine Utilization 2
Transport of Molybdenum 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
adenosine nucleotides degradation II 5 4 2
urate conversion to allantoin I 3 2 1
ureide biosynthesis 7 5 2
guanosine nucleotides degradation II 4 4 1
adenosine nucleotides degradation I 8 7 2
guanosine nucleotides degradation III 4 3 1
inosine 5'-phosphate degradation 4 3 1
guanosine nucleotides degradation I 4 3 1
allantoin degradation to ureidoglycolate II (ammonia producing) 4 1 1
allantoin degradation to glyoxylate II 5 1 1
allantoin degradation to glyoxylate III 5 1 1
purine nucleotides degradation II (aerobic) 11 8 2
purine nucleotides degradation I (plants) 12 10 2
superpathway of guanosine nucleotides degradation (plants) 6 5 1
superpathway of purines degradation in plants 18 12 3
purine nucleobases degradation II (anaerobic) 24 14 4
molybdopterin biosynthesis 6 3 1
caffeine degradation III (bacteria, via demethylation) 7 1 1
superpathway of allantoin degradation in plants 8 1 1
allantoin degradation IV (anaerobic) 9 3 1
theophylline degradation 9 1 1
caffeine degradation IV (bacteria, via demethylation and oxidation) 10 1 1