Experiment set2IT007 for Desulfovibrio vulgaris Miyazaki F

MoYLS4 with Sodium nitrite 1.25 mg/ml

Group: stress
Media: MoYLS4 + Sodium nitrite (1.25 mg/ml), pH=7.2
Culturing: Miya_ML3, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Jen on 11/23/2014
Media components: 1 g/L Yeast Extract, 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, 15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate, Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 3 genes in this experiment

For stress Sodium nitrite in Desulfovibrio vulgaris Miyazaki F

For stress Sodium nitrite across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	2
Nitrosative stress	1
Soluble cytochromes and functionally related electron carriers	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
nitrate reduction IV (dissimilatory)	2	1	1
formate to nitrite electron transfer	3	1	1