Experiment set2IT007 for Caulobacter crescentus NA1000

Adenosine nitrogen source

Group: nitrogen source
Media: M2_noNitrogen + Adenosine (2 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 5.1 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 20 mM D-Glucose, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1527 B4

Specific Phenotypes

For 17 genes in this experiment

For nitrogen source Adenosine in Caulobacter crescentus NA1000

For nitrogen source Adenosine across organisms

SEED Subsystems

Subsystem	#Specific
Molybdenum cofactor biosynthesis	3
Purine Utilization	2
Methionine Biosynthesis	1
Potassium homeostasis	1
Purine conversions	1
Transport of Molybdenum	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Methionine metabolism
• Cysteine metabolism
• gamma-Hexachlorocyclohexane degradation
• Fluorobenzoate degradation
• Selenoamino acid metabolism
• 1,4-Dichlorobenzene degradation
• Nitrogen metabolism
• Sulfur metabolism
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
adenosine nucleotides degradation II	5	4	3
adenine and adenosine salvage V	3	1	1
purine deoxyribonucleosides degradation II	3	1	1
purine nucleotides degradation II (aerobic)	11	8	3
guanosine nucleotides degradation II	4	4	1
adenosine nucleotides degradation I	8	7	2
guanosine nucleotides degradation III	4	3	1
inosine 5'-phosphate degradation	4	3	1
guanosine nucleotides degradation I	4	3	1
adenine and adenosine salvage III	4	2	1
purine deoxyribonucleosides degradation I	4	1	1
4-chlorocatechol degradation	5	2	1
3-chlorocatechol degradation II (ortho)	5	1	1
3-chlorocatechol degradation I (ortho)	5	1	1
purine nucleotides degradation I (plants)	12	10	2
superpathway of guanosine nucleotides degradation (plants)	6	5	1
purine nucleobases degradation II (anaerobic)	24	14	4
molybdopterin biosynthesis	6	3	1
hydrogen sulfide biosynthesis II (mammalian)	6	1	1
purine ribonucleosides degradation	6	1	1
ureide biosynthesis	7	5	1
4,5-dichlorocatechol degradation	7	3	1
caffeine degradation III (bacteria, via demethylation)	7	1	1
superpathway of purine deoxyribonucleosides degradation	7	1	1
3,5-dichlorocatechol degradation	8	3	1
superpathway of purines degradation in plants	18	12	2
3,4,6-trichlorocatechol degradation	9	4	1
1,4-dichlorobenzene degradation	9	3	1
theophylline degradation	9	1	1
caffeine degradation IV (bacteria, via demethylation and oxidation)	10	1	1