Experiment set2IT006 for Caulobacter crescentus NA1000

D-Glucosamine Hydrochloride nitrogen source

Group: nitrogen source
Media: M2_noNitrogen + D-Glucosamine Hydrochloride (20 mM)
Culturing: Caulo_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.8 generations
By: Jayashree on 22-Mar-17
Media components: 1.74 g/L Disodium phosphate, 1.06 g/L Potassium phosphate monobasic, 20 mM D-Glucose, 0.5 mM Magnesium sulfate, 0.5 mM Calcium chloride, 0.01 mM Iron (II) sulfate heptahydrate, 0.008 mM EDTA
Growth plate: 1527 B2

Specific Phenotypes

For 4 genes in this experiment

For nitrogen source D-Glucosamine Hydrochloride in Caulobacter crescentus NA1000

For nitrogen source D-Glucosamine Hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
Chitin and N-acetylglucosamine utilization	3
N-Acetyl-Galactosamine and Galactosamine Utilization	1
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Other glycan degradation
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Glycosaminoglycan degradation
• Glycosphingolipid biosynthesis - globo series
• Glycosphingolipid biosynthesis - ganglio series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
chitin degradation II (Vibrio)	6	3	2
chitin degradation III (Serratia)	7	3	1
peptidoglycan recycling II	10	2	1
peptidoglycan recycling I	14	8	1