Experiment set2IT005 for Pedobacter sp. GW460-11-11-14-LB5
R2A with D-Cycloserine 0.2 mg/ml
Group: stressMedia: R2A + D-Cycloserine (0.2 mg/ml)
Culturing: Pedo557_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.2 generations
By: Adam on 17-May-17
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Growth plate: 1759 C3
Specific Phenotypes
For 6 genes in this experiment
For stress D-Cycloserine in Pedobacter sp. GW460-11-11-14-LB5
For stress D-Cycloserine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glutathione-regulated potassium-efflux system and associated functions | 1 |
| Potassium homeostasis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| proline to cytochrome bo oxidase electron transfer | 2 | 1 | 1 |
| L-proline degradation I | 3 | 3 | 1 |
| (5R)-carbapenem carboxylate biosynthesis | 6 | 1 | 1 |
| L-Nδ-acetylornithine biosynthesis | 7 | 4 | 1 |
| L-citrulline biosynthesis | 8 | 6 | 1 |
| superpathway of L-citrulline metabolism | 12 | 8 | 1 |