Experiment set2H45 for Phaeobacter inhibens DSM 17395

marine broth with methylglyoxal 0.004 vol%

Group: stress
Media: marine_broth_2216 + methylglyoxal (0.004 vol%)
Culturing: Phaeo_ML1, 48 well microplate; Tecan Infinite F200, Aerobic, at 25 (C), shaken=orbital
By: Jordan on 8/5/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 616 B1,B2

Specific Phenotypes

For 13 genes in this experiment

For stress methylglyoxal in Phaeobacter inhibens DSM 17395

For stress methylglyoxal across organisms

SEED Subsystems

Subsystem	#Specific
DNA repair, UvrABC system	3
DNA repair, bacterial RecFOR pathway	3
DNA-replication	2
Cysteine Biosynthesis	1
DNA repair, bacterial	1
Glutathione: Non-redox reactions	1
Methylglyoxal Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyruvate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
methylglyoxal degradation VIII	3	3	1
methylglyoxal degradation I	3	2	1
superpathway of methylglyoxal degradation	8	3	1