Experiment set2H41 for Dechlorosoma suillum PS

Anaerobic growth with lactate carbon source, perchlorate electron acceptor

Group: anaerobic
Media: ALP_defined_noC + Sodium D,L-Lactate (30 mM) + Sodium perchlorate monohydrate (10 uM), pH=7.2
Culturing: PS_ML1, tube, Anaerobic, at 37 (C), shaken=200 rpm
By: Ryan Melnyk on 8/1/2013
Media components: 0.49 g/L Sodium phosphate monobasic monohydrate, 0.97 g/L Potassium phosphate dibasic, 0.1 g/L Potassium Chloride, 0.25 g/L Ammonium chloride, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 4 genes in this experiment

For anaerobic Sodium D,L-Lactate in Dechlorosoma suillum PS

For anaerobic Sodium D,L-Lactate across organisms

SEED Subsystems

Subsystem	#Specific
Flagellum	1
Glutathione: Biosynthesis and gamma-glutamyl cycle	1
Heat shock dnaK gene cluster extended	1
cAMP signaling in bacteria	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glutathione metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glutathione biosynthesis	2	2	1
ophthalmate biosynthesis	3	2	1
γ-glutamyl cycle	6	3	1