Experiment set2H28 for Marinobacter adhaerens HP15

marine broth with Sodium perchlorate monohydrate 100 mM

Group: stress
Media: marine_broth_2216 + Sodium perchlorate monohydrate (100 mM)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 9/9/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 681 E1,E2

Specific Phenotypes

For 8 genes in this experiment

For stress Sodium perchlorate monohydrate in Marinobacter adhaerens HP15

For stress Sodium perchlorate monohydrate across organisms

SEED Subsystems

Subsystem	#Specific
Cysteine Biosynthesis	3
Glutathione: Non-redox reactions	1
Stringent Response, (p)ppGpp metabolism	1
Sulfur oxidation	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Purine metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
ppGpp metabolism	6	6	2
molybdenum cofactor biosynthesis	3	1	1