Experiment set2H22 for Shewanella amazonensis SB2B
Minimal media with Sodium D,L-Lactate as carbon source
Group: carbon sourceMedia: ShewMM_noCarbon + Sodium D,L-Lactate (20 mM), pH=7
Culturing: SB2B_ML5, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Jake on 4/15/2013
Media components: 1.5 g/L Ammonium chloride, 1.75 g/L Sodium Chloride, 0.61 g/L Magnesium chloride hexahydrate, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: 505 B7,B8
Specific Phenotypes
For 12 genes in this experiment
For carbon source Sodium D,L-Lactate in Shewanella amazonensis SB2B
For carbon source Sodium D,L-Lactate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Alkylphosphonate utilization | 1 |
| Fatty Acid Biosynthesis FASII | 1 |
| Glutathione: Non-redox reactions | 1 |
| Synechocystis experimental | 1 |
| Triacylglycerol metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid biosynthesis
- Urea cycle and metabolism of amino groups
- Arginine and proline metabolism
- Glycerophospholipid metabolism
- Tetracycline biosynthesis
- Lysine degradation
- Histidine metabolism
- Tyrosine metabolism
- Phenylalanine metabolism
- Glutathione metabolism
- Lipopolysaccharide biosynthesis
- Ether lipid metabolism
- Glycosphingolipid biosynthesis - ganglio series
- Pyruvate metabolism
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Propanoate metabolism
- Ethylbenzene degradation
- Butanoate metabolism
- Atrazine degradation
- Limonene and pinene degradation
- Diterpenoid biosynthesis
- Carotenoid biosynthesis - General
- Anthocyanin biosynthesis
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Metabolism of xenobiotics by cytochrome P450
- Drug metabolism - cytochrome P450
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide backbone
- Biosynthesis of terpenoids and steroids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: