Experiment set2H18 for Marinobacter adhaerens HP15

Compare to:

marine broth with Paraquat dichloride 0.0075 mg/ml

Group: stress
Media: marine_broth_2216 + Paraquat dichloride (0.0075 mg/ml)
Culturing: Marino_ML2, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Jordan on 9/9/2013
Media components: 5 g/L Bacto Peptone, 1 g/L Yeast Extract, 0.1 g/L Ferric citrate, 19.45 g/L Sodium Chloride, 5.9 g/L Magnesium chloride hexahydrate, 3.24 g/L Magnesium sulfate, 1.8 g/L Calcium chloride, 0.55 g/L Potassium Chloride, 0.16 g/L Sodium bicarbonate, 0.08 g/L Potassium bromide, 34 mg/L Strontium chloride, 22 mg/L Boric Acid, 4 mg/L Sodium metasilicate, 2.4 mg/L sodium fluoride, 8 mg/L Disodium phosphate
Growth plate: 680 D3,D4

Specific Phenotypes

For 17 genes in this experiment

For stress Paraquat dichloride in Marinobacter adhaerens HP15

For stress Paraquat dichloride across organisms

SEED Subsystems

Subsystem #Specific
Choline and Betaine Uptake and Betaine Biosynthesis 3
Cysteine Biosynthesis 2
Campylobacter Iron Metabolism 1
Heat shock dnaK gene cluster extended 1
Iron acquisition in Vibrio 1
Protein chaperones 1
Thioredoxin-disulfide reductase 1
Transport of Iron 1
tRNA processing 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glycine betaine biosynthesis I (Gram-negative bacteria) 2 2 2
glycine betaine biosynthesis II (Gram-positive bacteria) 2 2 2
choline degradation I 2 2 2
choline-O-sulfate degradation 3 2 2
β-alanine biosynthesis I 2 1 1
β-alanine biosynthesis IV 2 1 1
glycine betaine biosynthesis III (plants) 3 2 1
molybdenum cofactor biosynthesis 3 1 1
dimethylsulfoniopropanoate biosynthesis I (Wollastonia) 3 1 1
choline degradation IV 4 2 1
dimethylsulfoniopropanoate biosynthesis II (Spartina) 4 1 1
tRNA processing 10 10 2