Experiment set28S498 for Bacteroides thetaiotaomicron VPI-5482

Compare to:

0.35mg/ml WT Calprotectin + 1uM saturated Fe-Enterobactin in 50% 50%BHI + 50% Calprotectin buffer + 1x protoporphyrin, cultured for 20 hours.

Group: stress
Media: BHIS (0.5x) + Calprotectin (0.35 mg/mL) + Enterobactin (1 uM) + 0.5X Calprotectin buffer + Protoporphyrin
Culturing: Btheta_ML6a, tube, Anaerobic, at 37 (C), shaken=0 rpm
By: Wenhan Zhu on 8/1/23
Media components: 3.85 g/L Calf brains, 4.9 g/L Beef heart, 5 g/L Proteose Peptone, 2.5 g/L Sodium Chloride, 1.25 g/L Disodium phosphate, 0.5 g/L L-Cysteine, 1 g/L Sodium bicarbonate, 0.0025 g/L Hemin (final concentrations)

Calprotectin buffer 0.5x includes: 10 mM Tris hydrochloride, 50 mM Sodium Chloride, 1.5 mM Calcium chloride dihydrate, 2.5 mM 2-Mercaptoethanol (final concentrations)

Specific Phenotypes

For 3 genes in this experiment

For stress Calprotectin in Bacteroides thetaiotaomicron VPI-5482

For stress Calprotectin across organisms

SEED Subsystems

Subsystem #Specific
Transport of Iron 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.