Experiment set28IT055 for Burkholderia phytofirmans PsJN
P. phyto corn MOI 1
Group: phageMedia: LB_plus_SM_buffer + Kan (50 ug/ml)
Culturing: BFirm_ML3a, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: Vivek on 12/15/22
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 2 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Rhamnose containing glycans | 1 |
| dTDP-rhamnose synthesis | 1 |
| linker unit-arabinogalactan synthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| dTDP-β-L-rhamnose biosynthesis | 5 | 5 | 1 |
| O-antigen building blocks biosynthesis (E. coli) | 11 | 11 | 1 |
| superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis | 19 | 5 | 1 |
| superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis | 33 | 13 | 1 |