Experiment set27S436 for Pseudomonas fluorescens SBW25-INTG

4-Guanidinobutyric acid carbon source

Group: carbon source
Media: MME_noCarbon + 4-Guanidinobutyric acid (10 mM)
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C)
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 7 genes in this experiment

For carbon source 4-Guanidinobutyric acid in Pseudomonas fluorescens SBW25-INTG

For carbon source 4-Guanidinobutyric acid across organisms

SEED Subsystems

Subsystem	#Specific
Arginine and Ornithine Degradation	1
DNA-binding regulatory proteins, strays	1
Homogentisate pathway of aromatic compound degradation	1
Lysine degradation	1
Polyamine Metabolism	1
Salicylate and gentisate catabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Glutamate metabolism
• Lysine degradation
• Tyrosine metabolism
• Butanoate metabolism
• Alanine and aspartate metabolism
• Geraniol degradation
• Lysine biosynthesis
• Tryptophan metabolism
• beta-Alanine metabolism
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• Propanoate metabolism
• Styrene degradation
• Limonene and pinene degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
4-aminobutanoate degradation III	2	2	2
putrescine biosynthesis I	2	2	1
4-aminobutanoate degradation II	2	2	1
4-aminobutanoate degradation I	2	2	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
L-arginine degradation XI	2	1	1
L-lysine degradation IV	5	5	2
superpathway of 4-aminobutanoate degradation	3	3	1
L-lysine degradation X	6	5	2
L-arginine degradation X (arginine monooxygenase pathway)	3	2	1
L-lysine degradation III	6	2	2
L-lysine degradation I	7	4	2
superpathway of putrescine biosynthesis	4	4	1
GABA shunt II	4	3	1
L-arginine degradation IX (arginine:pyruvate transaminase pathway)	4	2	1
GABA shunt I	4	2	1
L-arginine degradation VIII (arginine oxidase pathway)	4	2	1
L-arginine degradation XII	4	1	1
L-tyrosine degradation I	5	5	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	7	2
superpathway of L-arginine and L-ornithine degradation	13	9	2
4-aminobutanoate degradation V	7	3	1
superpathway of polyamine biosynthesis I	8	6	1
superpathway of arginine and polyamine biosynthesis	17	15	1
nicotine degradation I (pyridine pathway)	17	5	1
superpathway of L-lysine degradation	43	17	2