Experiment set27S416 for Pseudomonas fluorescens SBW25-INTG

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sn-glycero-3-phosphocholine carbon source

Group: carbon source
Media: MME_noCarbon + sn-glycero-3-phosphocholine (10 mM)
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C)
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 9 genes in this experiment

For carbon source sn-glycero-3-phosphocholine in Pseudomonas fluorescens SBW25-INTG

For carbon source sn-glycero-3-phosphocholine across organisms

SEED Subsystems

Subsystem #Specific
Choline and Betaine Uptake and Betaine Biosynthesis 2
Glycerol and Glycerol-3-phosphate Uptake and Utilization 2
Iron acquisition in Vibrio 2
High affinity phosphate transporter and control of PHO regulon 1
Phosphate metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glycine betaine biosynthesis II (Gram-positive bacteria) 2 2 2
glycine betaine biosynthesis I (Gram-negative bacteria) 2 2 2
choline degradation I 2 2 2
choline-O-sulfate degradation 3 3 2
glycerophosphodiester degradation 2 2 1
β-alanine biosynthesis IV 2 1 1
phosphatidylcholine resynthesis via glycerophosphocholine 2 1 1
β-alanine biosynthesis I 2 1 1
glycine betaine biosynthesis III (plants) 3 2 1
dimethylsulfoniopropanoate biosynthesis I (Wollastonia) 3 1 1
glycerol and glycerophosphodiester degradation 4 4 1
phospholipid remodeling (phosphatidylethanolamine, yeast) 4 2 1
choline degradation IV 4 2 1
dimethylsulfoniopropanoate biosynthesis II (Spartina) 4 1 1
anandamide biosynthesis I 12 3 1