Experiment set27S394 for Pseudomonas fluorescens SBW25-INTG
D,L-Malic Acid carbon source
Group: carbon sourceMedia: MME_noCarbon + D,L-Malic Acid (20 mM)
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C)
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 2 genes in this experiment
For carbon source D,L-Malic Acid in Pseudomonas fluorescens SBW25-INTG
For carbon source D,L-Malic Acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Bacterial Cytoskeleton | 1 |
| Plasmid replication | 1 |
| TCA Cycle | 1 |
| Two cell division clusters relating to chromosome partitioning | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| partial TCA cycle (obligate autotrophs) | 8 | 8 | 1 |
| TCA cycle VII (acetate-producers) | 9 | 7 | 1 |
| TCA cycle VI (Helicobacter) | 9 | 7 | 1 |
| TCA cycle I (prokaryotic) | 10 | 9 | 1 |
| superpathway of glyoxylate bypass and TCA | 12 | 11 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 22 | 1 |