Experiment set27S387 for Pseudomonas fluorescens SBW25-INTG

Sodium L-Lactate carbon source

Group: carbon source
Media: MME_noCarbon + Sodium L-Lactate (20 mM)
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C)
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source Sodium L-Lactate in Pseudomonas fluorescens SBW25-INTG

For carbon source Sodium L-Lactate across organisms

SEED Subsystems

Subsystem	#Specific
Lactate utilization	2
Bacterial Cytoskeleton	1
L-rhamnose utilization	1
Murein hydrolase regulation and cell death	1
Plasmid replication	1
Respiratory dehydrogenases 1	1
Two cell division clusters relating to chromosome partitioning	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyruvate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-lactaldehyde degradation (aerobic)	2	2	1
methylglyoxal degradation V	3	2	1
methylglyoxal degradation IV	3	2	1
superpathway of methylglyoxal degradation	8	5	1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales)	8	4	1
superpathway of fucose and rhamnose degradation	12	5	1