Experiment set27S330 for Bacteroides thetaiotaomicron VPI-5482
carbon source a1-Acid Glycoprotein from bovine plasma 10 mg/ml
Group: carbon sourceMedia: Varel_Bryant_medium + a1-Acid Glycoprotein from bovine plasma (10 mg/ml)
Culturing: Btheta_ML6a, tube, Anaerobic, at 37 (C), shaken=0 rpm
By: Didier Ndeh on 10/7/23
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 7 genes in this experiment
For carbon source a1-Acid Glycoprotein from bovine plasma in Bacteroides thetaiotaomicron VPI-5482
For carbon source a1-Acid Glycoprotein from bovine plasma across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Carboxysome | 1 |
| Galactosylceramide and Sulfatide metabolism | 1 |
| Predicted carbohydrate hydrolases | 1 |
| Respiratory dehydrogenases 1 | 1 |
| Sialic Acid Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NADH to cytochrome bo oxidase electron transfer I | 2 | 1 | 1 |
| NADH to cytochrome bd oxidase electron transfer I | 2 | 1 | 1 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 3 | 1 |
| aerobic respiration III (alternative oxidase pathway) | 3 | 2 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 2 | 1 |
| NAD(P)/NADPH interconversion | 6 | 2 | 1 |
| Fe(II) oxidation | 6 | 1 | 1 |