Experiment set27S308 for Bacteroides thetaiotaomicron VPI-5482
carbon source Rhamnogalacturonan-II from apple juice concentrate 10 mg/ml
Group: carbon sourceMedia: Varel_Bryant_medium + Rhamnogalacturonan-II from apple juice concentrate (10 mg/ml)
Culturing: Btheta_ML6a, tube, Anaerobic, at 37 (C), shaken=0 rpm
By: Didier Ndeh on 10/7/23
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 2 genes in this experiment
For carbon source Rhamnogalacturonan-II from apple juice concentrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Carboxysome | 1 |
| Respiratory dehydrogenases 1 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NADH to cytochrome bo oxidase electron transfer I | 2 | 1 | 1 |
| NADH to cytochrome bd oxidase electron transfer I | 2 | 1 | 1 |
| aerobic respiration III (alternative oxidase pathway) | 3 | 2 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 2 | 1 |
| NAD(P)/NADPH interconversion | 6 | 2 | 1 |
| Fe(II) oxidation | 6 | 1 | 1 |