Experiment set26S517 for Burkholderia phytofirmans PsJN

Compare to:

Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50

200 most detrimental genes:

  gene name fitness t score description  
BPHYT_RS27920 +1.7 1.7 tricarballylate utilization protein B compare
BPHYT_RS21625 +1.6 1.7 LysR family transcriptional regulator compare
BPHYT_RS25800 +1.6 3.2 sugar ABC transporter permease compare
BPHYT_RS28535 +1.5 1.2 response regulator receiver compare
BPHYT_RS03015 +1.4 1.6 aldolase compare
BPHYT_RS19985 +1.4 1.3 CoA-transferase compare
BPHYT_RS27175 +1.3 1.2 DNA-binding protein compare
BPHYT_RS14060 +1.3 2.4 peptidase M23 compare
BPHYT_RS30365 +1.3 1.7 beta-lactamase compare
BPHYT_RS33905 +1.3 2.0 3-isopropylmalate dehydratase small subunit compare
BPHYT_RS01435 +1.3 11.8 LysR family transcriptional regulator compare
BPHYT_RS15890 +1.2 1.4 3-hydroxy-2-methylbutyryl-CoA dehydrogenase compare
BPHYT_RS21470 +1.2 2.7 6-hydroxynicotinate reductase compare
BPHYT_RS04015 +1.2 2.5 glycosyl transferase compare
BPHYT_RS26820 +1.2 1.2 LysR family transcriptional regulator compare
BPHYT_RS07935 +1.2 2.2 Rrf2 family transcriptional regulator compare
BPHYT_RS08150 +1.2 1.0 membrane protein compare
BPHYT_RS28075 +1.2 1.1 cytochrome B561 compare
BPHYT_RS22835 +1.2 1.3 flagellar motor protein MotA compare
BPHYT_RS28660 +1.1 1.2 polyvinylalcohol dehydrogenase compare
BPHYT_RS05395 +1.1 1.8 hypothetical protein compare
BPHYT_RS09690 +1.1 1.4 phosphate starvation-inducible protein PsiF compare
BPHYT_RS18835 +1.1 1.8 flagellar biosynthesis protein FliR compare
BPHYT_RS32225 +1.1 1.5 hypothetical protein compare
BPHYT_RS07660 +1.1 2.3 sulfonate ABC transporter substrate-binding protein compare
BPHYT_RS28830 +1.1 1.1 LysR family transcriptional regulator compare
BPHYT_RS35275 +1.1 1.9 acetyltransferase compare
BPHYT_RS30635 +1.1 2.4 D-aminopeptidase compare
BPHYT_RS23030 +1.1 1.4 gluconate 2-dehydrogenase compare
BPHYT_RS13915 +1.0 2.5 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase compare
BPHYT_RS27065 +1.0 1.3 hypothetical protein compare
BPHYT_RS10165 +1.0 1.2 transcriptional regulator compare
BPHYT_RS23940 +1.0 0.8 GntR family transcriptional regulator compare
BPHYT_RS18420 +1.0 2.1 hypothetical protein compare
BPHYT_RS02570 +1.0 6.4 phosphocarrier protein HPr compare
BPHYT_RS16065 +1.0 0.7 LacI family transcriptional regulator compare
BPHYT_RS03875 +1.0 2.0 dehydrogenase compare
BPHYT_RS29365 +1.0 1.3 ATP-NAD kinase compare
BPHYT_RS22125 +1.0 1.6 hypothetical protein compare
BPHYT_RS10260 +0.9 1.5 hypothetical protein compare
BPHYT_RS27720 +0.9 1.1 hypothetical protein compare
BPHYT_RS22815 +0.9 1.0 TonB-denpendent receptor compare
BPHYT_RS24580 +0.9 0.9 hypothetical protein compare
BPHYT_RS10710 +0.9 1.9 sensor histidine kinase compare
BPHYT_RS01120 +0.9 2.7 plasmid partitioning protein ParB compare
BPHYT_RS08135 +0.9 1.3 hypothetical protein compare
BPHYT_RS34100 +0.9 1.8 spermidine/putrescine ABC transporter ATP-binding protein compare
BPHYT_RS33515 +0.9 7.1 porin compare
BPHYT_RS15775 +0.9 1.5 peptide ABC transporter permease compare
BPHYT_RS11260 +0.9 1.4 LysR family transcriptional regulator compare
BPHYT_RS24665 +0.9 1.6 ABC transporter permease compare
BPHYT_RS12265 +0.9 0.7 alanine acetyltransferase compare
BPHYT_RS28190 +0.9 1.3 porin compare
BPHYT_RS02120 +0.9 1.7 hypothetical protein compare
BPHYT_RS10705 +0.9 1.8 MFS transporter compare
BPHYT_RS16055 +0.9 2.2 sugar ABC transporter permease compare
BPHYT_RS24350 +0.8 1.7 hypothetical protein compare
BPHYT_RS27410 +0.8 0.7 amidohydrolase compare
BPHYT_RS05290 +0.8 0.5 hypothetical protein compare
BPHYT_RS09345 +0.8 1.3 TetR family transcriptional regulator compare
BPHYT_RS07595 +0.8 1.2 membrane protein compare
BPHYT_RS09765 +0.8 1.6 G-D-S-L family lipolytic protein compare
BPHYT_RS25560 +0.8 1.0 RNA polymerase sigma factor compare
BPHYT_RS31260 +0.8 1.2 histidine kinase compare
BPHYT_RS19565 +0.8 7.0 gamma-glutamyl:cysteine ligase compare
BPHYT_RS03625 +0.8 4.8 Histidinol-phosphatase (EC:3.1.3.15) (from data) compare
BPHYT_RS22455 +0.8 0.8 phosphonate ABC transporter substrate-binding protein compare
BPHYT_RS25950 +0.8 2.6 sensor histidine kinase compare
BPHYT_RS29120 +0.8 2.3 transcriptional regulator compare
BPHYT_RS29475 +0.8 1.3 carbon-phosphorus lyase complex subunit PhnJ compare
BPHYT_RS25005 +0.8 0.8 FMN reductase compare
BPHYT_RS16855 +0.8 5.3 16S rRNA methyltransferase compare
BPHYT_RS23955 +0.8 2.1 LysR family transcriptional regulator compare
BPHYT_RS28740 +0.8 2.0 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase compare
BPHYT_RS15035 +0.8 2.7 hypothetical protein compare
BPHYT_RS09545 +0.8 1.6 peptide methionine sulfoxide reductase compare
BPHYT_RS04710 +0.8 1.1 hypothetical protein compare
BPHYT_RS05850 +0.8 1.5 hypothetical protein compare
BPHYT_RS23615 +0.8 0.9 XRE family transcriptional regulator compare
BPHYT_RS33385 +0.8 1.2 major facilitator superfamily protein compare
BPHYT_RS09465 +0.8 0.9 transcriptional regulator compare
BPHYT_RS14950 +0.8 1.9 hypothetical protein compare
BPHYT_RS23605 +0.8 1.5 hypothetical protein compare
BPHYT_RS22045 +0.8 1.5 peptide ABC transporter permease compare
BPHYT_RS06240 +0.8 2.7 hypothetical protein compare
BPHYT_RS11040 +0.8 0.7 GntR family transcriptional regulator compare
BPHYT_RS28735 +0.8 2.2 hypothetical protein compare
BPHYT_RS09365 +0.8 1.0 cytochrome C2 compare
BPHYT_RS17735 +0.8 2.2 ABC transporter ATP-binding protein compare
BPHYT_RS01170 +0.8 1.4 hypothetical protein compare
BPHYT_RS20290 +0.8 1.2 LysR family transcriptional regulator compare
BPHYT_RS10465 +0.8 1.4 hypothetical protein compare
BPHYT_RS30885 +0.8 2.1 hypothetical protein compare
BPHYT_RS08765 +0.8 0.6 ABC transporter ATP-binding protein compare
BPHYT_RS34500 +0.8 2.5 hypothetical protein compare
BPHYT_RS16495 +0.8 4.0 glycerol kinase compare
BPHYT_RS26105 +0.7 1.4 hypothetical protein compare
BPHYT_RS03185 +0.7 3.1 thiopurine S-methyltransferase compare
BPHYT_RS04430 +0.7 1.2 amino acid ABC transporter ATPase compare
BPHYT_RS16665 +0.7 2.2 peroxiredoxin compare
BPHYT_RS15515 +0.7 1.9 D-aminopeptidase compare
BPHYT_RS19885 +0.7 3.6 hypothetical protein compare
BPHYT_RS13895 +0.7 1.3 sugar ABC transporter substrate-binding protein compare
BPHYT_RS18910 +0.7 1.1 FlgN compare
BPHYT_RS19570 +0.7 6.7 sodium/hydrogen exchanger compare
BPHYT_RS30800 +0.7 1.1 MFS transporter compare
BPHYT_RS32540 +0.7 1.5 hypothetical protein compare
BPHYT_RS00750 +0.7 1.5 SpoVT/AbrB domain-containing protein compare
BPHYT_RS01660 +0.7 2.4 hypothetical protein compare
BPHYT_RS09970 +0.7 1.4 glutathione S-transferase compare
BPHYT_RS22820 +0.7 1.2 hydrolase compare
BPHYT_RS33820 +0.7 5.1 histidine kinase compare
BPHYT_RS18415 +0.7 2.6 hypothetical protein compare
BPHYT_RS01530 +0.7 1.2 penicillin-binding protein 2 compare
BPHYT_RS09880 +0.7 1.5 MFS transporter compare
BPHYT_RS27625 +0.7 1.5 transport-associated protein compare
BPHYT_RS33535 +0.7 5.9 RNA polymerase sigma factor RpoD compare
BPHYT_RS29975 +0.7 1.7 multidrug ABC transporter ATP-binding protein compare
BPHYT_RS25100 +0.7 1.8 serine dehydratase compare
BPHYT_RS04060 +0.7 1.1 glycosyl transferase compare
BPHYT_RS05520 +0.7 1.6 transcriptional regulator compare
BPHYT_RS10765 +0.7 1.3 amidase compare
BPHYT_RS30750 +0.7 0.8 GntR family transcriptional regulator compare
BPHYT_RS24690 +0.7 4.6 ionic transporter y4hA compare
BPHYT_RS26950 +0.7 3.8 MFS transporter compare
BPHYT_RS28370 +0.7 0.8 peroxidase compare
BPHYT_RS28255 +0.7 0.7 hypothetical protein compare
BPHYT_RS23385 +0.7 1.1 group 1 glycosyl transferase compare
BPHYT_RS00860 +0.7 1.6 3-ketoacyl-ACP reductase compare
BPHYT_RS03620 +0.7 4.1 poly(A) polymerase compare
BPHYT_RS36145 +0.7 2.2 hypothetical protein compare
BPHYT_RS29015 +0.7 0.7 RND transporter compare
BPHYT_RS34280 +0.7 1.6 hypothetical protein compare
BPHYT_RS25070 +0.7 0.9 choline ABC transporter permease compare
BPHYT_RS35655 +0.7 1.1 hypothetical protein compare
BPHYT_RS31635 +0.7 1.5 choline dehydrogenase compare
BPHYT_RS32750 +0.7 0.9 lipoprotein compare
BPHYT_RS21485 +0.7 0.5 hypothetical protein compare
BPHYT_RS23600 +0.7 1.4 hypothetical protein compare
BPHYT_RS23860 +0.7 1.1 malonic semialdehyde reductase compare
BPHYT_RS05065 +0.7 2.1 DNA mismatch repair protein MutS compare
BPHYT_RS32675 +0.7 1.5 2-methylcitrate dehydratase compare
BPHYT_RS02880 +0.7 1.0 ATP-dependent protease compare
BPHYT_RS25875 +0.7 0.7 type III secretion protein compare
BPHYT_RS06510 +0.7 1.4 DeoR faimly transcriptional regulator compare
BPHYT_RS26965 +0.7 0.7 L-lactate dehydrogenase, LldG subunit (from data) compare
BPHYT_RS06385 +0.7 2.6 ribosomal RNA large subunit methyltransferase H compare
BPHYT_RS27200 +0.7 2.1 pyridoxal 4-dehydrogenase compare
BPHYT_RS10860 +0.7 1.7 transcriptional regulator compare
BPHYT_RS31205 +0.7 0.9 peptide ABC transporter ATP-binding protein compare
BPHYT_RS35725 +0.7 1.2 hypothetical protein compare
BPHYT_RS13040 +0.7 1.3 hypothetical protein compare
BPHYT_RS06435 +0.7 0.8 hypothetical protein compare
BPHYT_RS09580 +0.6 1.3 2-aminoadipate aminotransferase compare
BPHYT_RS02595 +0.6 0.9 sulfurtransferase compare
BPHYT_RS24670 +0.6 1.8 ABC transporter permease compare
BPHYT_RS05830 +0.6 3.1 hypothetical protein compare
BPHYT_RS05505 +0.6 2.2 histidine/lysine/arginine/ornithine ABC transporter permease HisQ compare
BPHYT_RS06070 +0.6 2.9 cytochrome C compare
BPHYT_RS11310 +0.6 0.5 LysR family transcriptional regulator compare
BPHYT_RS25845 +0.6 0.7 RND transporter MFP subunit compare
BPHYT_RS35345 +0.6 2.2 decarboxylase UbiD compare
BPHYT_RS12635 +0.6 1.7 translation factor Sua5 compare
BPHYT_RS29260 +0.6 2.9 esterase compare
BPHYT_RS27450 +0.6 1.4 alpha/beta hydrolase compare
BPHYT_RS26290 +0.6 1.8 photosystem reaction center subunit H compare
BPHYT_RS32290 +0.6 1.4 hypothetical protein compare
BPHYT_RS32935 +0.6 2.7 3-ketoacyl-ACP reductase compare
BPHYT_RS26915 +0.6 1.9 Fis family transcriptional regulator compare
BPHYT_RS17725 +0.6 3.1 BolA family transcriptional regulator compare
BPHYT_RS00115 +0.6 1.2 acetaldehyde dehydrogenase compare
BPHYT_RS14050 +0.6 1.8 UDP pyrophosphate phosphatase compare
BPHYT_RS20220 +0.6 0.8 antibiotic synthesis protein MbtH compare
BPHYT_RS20355 +0.6 0.8 ABC transporter substrate-binding protein compare
BPHYT_RS05610 +0.6 4.1 DNA-cytosine methyltransferase compare
BPHYT_RS03655 +0.6 0.9 membrane protein compare
BPHYT_RS24810 +0.6 1.4 hypothetical protein compare
BPHYT_RS09100 +0.6 2.6 stationary phase survival protein SurE compare
BPHYT_RS16815 +0.6 1.3 ureidoglycolate hydrolase compare
BPHYT_RS28575 +0.6 0.5 transcriptional regulator compare
BPHYT_RS00815 +0.6 0.8 hypothetical protein compare
BPHYT_RS12160 +0.6 1.8 peptide deformylase compare
BPHYT_RS03375 +0.6 1.0 NAD(P) transhydrogenase compare
BPHYT_RS16445 +0.6 0.7 RNA helicase compare
BPHYT_RS05835 +0.6 1.9 hypothetical protein compare
BPHYT_RS32725 +0.6 2.0 cobalt transporter compare
BPHYT_RS00705 +0.6 1.2 branched-chain amino acid ABC transporter substrate-binding protein compare
BPHYT_RS28835 +0.6 1.6 3-(2-hydroxyphenyl) propionic acid transporter compare
BPHYT_RS20775 +0.6 1.0 3-ketoacyl-ACP reductase compare
BPHYT_RS21315 +0.6 1.6 hypothetical protein compare
BPHYT_RS35945 +0.6 1.8 response regulator receiver compare
BPHYT_RS32525 +0.6 0.6 hypothetical protein compare
BPHYT_RS04115 +0.6 2.7 glycosyhydrolase compare
BPHYT_RS11215 +0.6 3.7 ribose ABC transporter ATPase compare
BPHYT_RS30265 +0.6 0.7 hypothetical protein compare
BPHYT_RS25405 +0.6 1.5 CoA-transferase compare
BPHYT_RS36140 +0.6 2.5 hypothetical protein compare
BPHYT_RS04990 +0.6 0.7 hypothetical protein compare
BPHYT_RS32690 +0.6 1.7 acyl-CoA dehydrogenase compare
BPHYT_RS02300 +0.6 0.8 acid shock protein compare


Specific Phenotypes

None in this experiment

For Burkholderia phytofirmans PsJN in replicable vector efficiency experiments