Experiment set25S7 for Pseudomonas fluorescens SBW25-INTG

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D-Fructose carbon source

Group: carbon source
Media: MME_noCarbon + D-Fructose (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 31-Jan-23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 6 genes in this experiment

For carbon source D-Fructose in Pseudomonas fluorescens SBW25-INTG

For carbon source D-Fructose across organisms

SEED Subsystems

Subsystem #Specific
Fructose utilization 3
Mannitol Utilization 2
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization 2
Fructose and Mannose Inducible PTS 1
Sucrose utilization 1
Sucrose utilization Shewanella 1
Xylose utilization 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
D-sorbitol degradation I 3 3 2
sucrose degradation I (sucrose phosphotransferase) 3 2 1
sucrose degradation III (sucrose invertase) 4 3 1
sucrose degradation IV (sucrose phosphorylase) 4 3 1
D-altritol and galactitol degradation 4 1 1
sucrose degradation VII (sucrose 3-dehydrogenase) 4 1 1
sucrose degradation II (sucrose synthase) 5 4 1
mannitol cycle 5 3 1
heterolactic fermentation 18 13 1
superpathway of anaerobic sucrose degradation 19 14 1