Experiment set25S61 for Pseudomonas fluorescens SBW25-INTG
Glycerol carbon source
Group: carbon sourceMedia: MME_noCarbon + Glycerol (20 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 1 genes in this experiment
For carbon source Glycerol in Pseudomonas fluorescens SBW25-INTG
For carbon source Glycerol across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Conserved gene cluster possibly involved in RNA metabolism | 1 |
| Cysteine Biosynthesis | 1 |
| Methionine Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-cysteine biosynthesis I | 2 | 2 | 1 |
| L-cysteine biosynthesis VII (from S-sulfo-L-cysteine) | 4 | 3 | 1 |
| N-3-oxalyl-L-2,3-diaminopropanoate biosynthesis | 4 | 1 | 1 |
| seleno-amino acid biosynthesis (plants) | 5 | 3 | 1 |
| D-cycloserine biosynthesis | 6 | 1 | 1 |
| L-cysteine biosynthesis VI (reverse transsulfuration) | 7 | 3 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 9 | 1 |
| superpathway of seleno-compound metabolism | 19 | 8 | 1 |