Experiment set25S58 for Pseudomonas fluorescens SBW25-INTG
L-pyroglutamic acid carbon source
Group: carbon sourceMedia: MME_noCarbon + L-pyroglutamic acid (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 1/31/23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 6 genes in this experiment
For carbon source L-pyroglutamic acid in Pseudomonas fluorescens SBW25-INTG
For carbon source L-pyroglutamic acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Cyanate hydrolysis | 1 |
| DNA-binding regulatory proteins, strays | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| urea degradation I | 3 | 2 | 2 |
| cyanuric acid degradation II | 5 | 3 | 2 |
| cyanuric acid degradation I | 5 | 2 | 2 |
| L-citrulline degradation | 3 | 3 | 1 |
| superpathway of allantoin degradation in yeast | 6 | 4 | 2 |
| cyanate degradation | 3 | 2 | 1 |
| L-arginine degradation V (arginine deiminase pathway) | 4 | 4 | 1 |
| superpathway of atrazine degradation | 8 | 3 | 2 |
| uracil degradation III | 5 | 2 | 1 |
| allantoin degradation IV (anaerobic) | 9 | 2 | 1 |