Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
| Pathway | #Steps | #Present | #Specific |
|---|
| 2-oxoisovalerate decarboxylation to isobutanoyl-CoA | 3 | 3 | 3 |
| L-methionine degradation II | 3 | 3 | 2 |
| L-threonine degradation I | 6 | 4 | 4 |
| 2-methylcitrate cycle I | 5 | 5 | 3 |
| β-alanine degradation II | 2 | 2 | 1 |
| acetate and ATP formation from acetyl-CoA I | 2 | 2 | 1 |
| 2-methylcitrate cycle II | 6 | 5 | 3 |
| sulfoacetaldehyde degradation I | 2 | 1 | 1 |
| L-threonine degradation V | 2 | 1 | 1 |
| β-alanine degradation I | 2 | 1 | 1 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 3 |
| propanoyl-CoA degradation II | 5 | 4 | 2 |
| L-valine degradation I | 8 | 6 | 3 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 11 | 5 |
| benzoyl-CoA biosynthesis | 3 | 3 | 1 |
| 2-oxoglutarate decarboxylation to succinyl-CoA | 3 | 3 | 1 |
| pyruvate decarboxylation to acetyl CoA I | 3 | 3 | 1 |
| glycine cleavage | 3 | 3 | 1 |
| L-arginine degradation I (arginase pathway) | 3 | 3 | 1 |
| superpathway of acetate utilization and formation | 3 | 3 | 1 |
| L-proline biosynthesis III (from L-ornithine) | 3 | 3 | 1 |
| glycine biosynthesis II | 3 | 3 | 1 |
| L-ornithine biosynthesis II | 3 | 3 | 1 |
| pyruvate fermentation to acetate II | 3 | 3 | 1 |
| glyoxylate cycle | 6 | 5 | 2 |
| pyruvate fermentation to acetate I | 3 | 2 | 1 |
| pyruvate fermentation to acetate VII | 3 | 2 | 1 |
| pyruvate fermentation to acetate IV | 3 | 2 | 1 |
| partial TCA cycle (obligate autotrophs) | 8 | 8 | 2 |
| L-arginine degradation VI (arginase 2 pathway) | 4 | 4 | 1 |
| L-histidine degradation I | 4 | 4 | 1 |
| nitrogen remobilization from senescing leaves | 8 | 6 | 2 |
| pyruvate fermentation to acetate and (S)-lactate I | 4 | 3 | 1 |
| pyruvate fermentation to acetate and lactate II | 4 | 2 | 1 |
| sulfolactate degradation II | 4 | 1 | 1 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 3 |
| TCA cycle V (2-oxoglutarate synthase) | 9 | 7 | 2 |
| TCA cycle VI (Helicobacter) | 9 | 7 | 2 |
| TCA cycle VII (acetate-producers) | 9 | 7 | 2 |
| TCA cycle II (plants and fungi) | 9 | 7 | 2 |
| TCA cycle IV (2-oxoglutarate decarboxylase) | 9 | 7 | 2 |
| superpathway of L-threonine metabolism | 18 | 11 | 4 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 12 | 3 |
| hypoglycin biosynthesis | 14 | 4 | 3 |
| L-ornithine biosynthesis I | 5 | 5 | 1 |
| adipate degradation | 5 | 5 | 1 |
| L-arginine degradation II (AST pathway) | 5 | 5 | 1 |
| L-histidine degradation II | 5 | 5 | 1 |
| L-arginine degradation XIII (reductive Stickland reaction) | 5 | 5 | 1 |
| TCA cycle I (prokaryotic) | 10 | 9 | 2 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 4 | 1 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 4 | 1 |
| ethanolamine utilization | 5 | 4 | 1 |
| adipate biosynthesis | 5 | 4 | 1 |
| TCA cycle III (animals) | 10 | 7 | 2 |
| acetylene degradation (anaerobic) | 5 | 3 | 1 |
| acrylate degradation I | 5 | 3 | 1 |
| ferrichrome A biosynthesis | 5 | 2 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 2 | 1 |
| (S)-propane-1,2-diol degradation | 5 | 2 | 1 |
| mixed acid fermentation | 16 | 11 | 3 |
| reductive TCA cycle I | 11 | 6 | 2 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 2 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 3 |
| superpathway of glyoxylate bypass and TCA | 12 | 11 | 2 |
| β-alanine biosynthesis II | 6 | 5 | 1 |
| L-leucine degradation I | 6 | 5 | 1 |
| methyl ketone biosynthesis (engineered) | 6 | 4 | 1 |
| L-histidine degradation III | 6 | 4 | 1 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 1 |
| reductive TCA cycle II | 12 | 5 | 2 |
| superpathway of sulfolactate degradation | 6 | 2 | 1 |
| superpathway of taurine degradation | 6 | 2 | 1 |
| methanogenesis from acetate | 6 | 2 | 1 |
| L-Nδ-acetylornithine biosynthesis | 7 | 6 | 1 |
| fatty acid β-oxidation I (generic) | 7 | 6 | 1 |
| myo-inositol degradation I | 7 | 6 | 1 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 5 | 1 |
| acetyl-CoA fermentation to butanoate | 7 | 3 | 1 |
| 2,4-dinitrotoluene degradation | 7 | 3 | 1 |
| benzoyl-CoA degradation I (aerobic) | 7 | 3 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 18 | 3 |
| L-citrulline biosynthesis | 8 | 8 | 1 |
| L-histidine degradation VI | 8 | 7 | 1 |
| lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) | 8 | 4 | 1 |
| L-arginine biosynthesis I (via L-ornithine) | 9 | 9 | 1 |
| L-lysine biosynthesis I | 9 | 9 | 1 |
| L-arginine biosynthesis III (via N-acetyl-L-citrulline) | 9 | 8 | 1 |
| flavin biosynthesis I (bacteria and plants) | 9 | 8 | 1 |
| flavin biosynthesis III (fungi) | 9 | 7 | 1 |
| valproate β-oxidation | 9 | 6 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 1 |
| superpathway of fermentation (Chlamydomonas reinhardtii) | 9 | 4 | 1 |
| phenylacetate degradation I (aerobic) | 9 | 4 | 1 |
| superpathway of L-alanine fermentation (Stickland reaction) | 9 | 4 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 1 |
| methylaspartate cycle | 19 | 9 | 2 |
| L-arginine biosynthesis II (acetyl cycle) | 10 | 10 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 9 | 1 |
| myo-, chiro- and scyllo-inositol degradation | 10 | 6 | 1 |
| flavin biosynthesis II (archaea) | 10 | 5 | 1 |
| 3-phenylpropanoate degradation | 10 | 5 | 1 |
| L-lysine fermentation to acetate and butanoate | 10 | 3 | 1 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 1 |
| superpathway of phenylethylamine degradation | 11 | 6 | 1 |
| gallate degradation III (anaerobic) | 11 | 3 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 4 | 2 |
| oleate β-oxidation | 35 | 33 | 3 |
| superpathway of L-citrulline metabolism | 12 | 10 | 1 |
| ethene biosynthesis V (engineered) | 25 | 18 | 2 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 22 | 2 |
| (S)-lactate fermentation to propanoate, acetate and hydrogen | 13 | 4 | 1 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 3 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 3 | 1 |
| cyclosporin A biosynthesis | 15 | 2 | 1 |
| superpathway of arginine and polyamine biosynthesis | 17 | 15 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 7 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 16 | 1 |
| heterolactic fermentation | 18 | 13 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 13 | 1 |
| superpathway of methanogenesis | 21 | 2 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 14 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 1 |
| aspartate superpathway | 25 | 22 | 1 |
| platensimycin biosynthesis | 26 | 6 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |
| superpathway of L-lysine degradation | 43 | 17 | 1 |