Experiment set25S26 for Pseudomonas fluorescens SBW25-INTG

m-Inositol carbon source

Group: carbon source
Media: MME_noCarbon + m-Inositol (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 31-Jan-23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 18 genes in this experiment

For carbon source m-Inositol in Pseudomonas fluorescens SBW25-INTG

For carbon source m-Inositol across organisms

SEED Subsystems

Subsystem	#Specific
Inositol catabolism	10
Acetyl-CoA fermentation to Butyrate	1
Anaerobic respiratory reductases	1
Butanol Biosynthesis	1
De Novo Pyrimidine Synthesis	1
Isobutyryl-CoA to Propionyl-CoA Module	1
Isoleucine degradation	1
LMPTP YwlE cluster	1
Valine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Inositol phosphate metabolism
• Valine, leucine and isoleucine degradation
• Fatty acid metabolism
• Propanoate metabolism
• Pentose and glucuronate interconversions
• Galactose metabolism
• Ascorbate and aldarate metabolism
• Pyrimidine metabolism
• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• Phenylalanine metabolism
• Benzoate degradation via hydroxylation
• beta-Alanine metabolism
• Streptomycin biosynthesis
• Biphenyl degradation
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Butanoate metabolism
• Nicotinate and nicotinamide metabolism
• Limonene and pinene degradation
• Caprolactam degradation
• Biosynthesis of ansamycins

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyrimidine nucleobases salvage I	1	1	1
myo-inositol degradation I	7	6	6
myo-, chiro- and scyllo-inositol degradation	10	6	6
β-alanine degradation II	2	2	1
pyrimidine nucleobases salvage II	2	2	1
β-alanine degradation I	2	1	1
superpathway of pyrimidine nucleobases salvage	4	4	1
propanoyl-CoA degradation II	5	4	1
acrylate degradation I	5	3	1
myo-inositol degradation II	5	1	1
pyruvate fermentation to butanol II (engineered)	6	4	1
2-methyl-branched fatty acid β-oxidation	14	11	2
2,4-dinitrotoluene degradation	7	3	1
L-valine degradation I	8	6	1
superpathway of pyrimidine ribonucleosides salvage	10	6	1
pyruvate fermentation to hexanol (engineered)	11	7	1
streptomycin biosynthesis	18	2	1
1-butanol autotrophic biosynthesis (engineered)	27	19	1