Experiment set25S25 for Pseudomonas fluorescens SBW25-INTG
m-Inositol carbon source
Group: carbon sourceMedia: MME_noCarbon + m-Inositol (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Andrew Frank on 31-Jan-23
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 10 mM Ammonium chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 13 genes in this experiment
For carbon source m-Inositol in Pseudomonas fluorescens SBW25-INTG
For carbon source m-Inositol across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Inositol catabolism | 9 |
| cAMP signaling in bacteria | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Inositol phosphate metabolism
- Pentose and glucuronate interconversions
- Galactose metabolism
- Ascorbate and aldarate metabolism
- Purine metabolism
- Valine, leucine and isoleucine degradation
- 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
- Phenylalanine metabolism
- Benzoate degradation via hydroxylation
- Streptomycin biosynthesis
- Biphenyl degradation
- Benzoate degradation via CoA ligation
- Propanoate metabolism
- Ethylbenzene degradation
- Nicotinate and nicotinamide metabolism
- Limonene and pinene degradation
- Caprolactam degradation
- Biosynthesis of ansamycins
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| myo-inositol degradation I | 7 | 6 | 6 |
| myo-, chiro- and scyllo-inositol degradation | 10 | 6 | 6 |
| β-alanine degradation II | 2 | 2 | 1 |
| β-alanine degradation I | 2 | 1 | 1 |
| propanoyl-CoA degradation II | 5 | 4 | 1 |
| acrylate degradation I | 5 | 3 | 1 |
| myo-inositol degradation II | 5 | 1 | 1 |
| 2,4-dinitrotoluene degradation | 7 | 3 | 1 |
| L-valine degradation I | 8 | 6 | 1 |
| streptomycin biosynthesis | 18 | 2 | 1 |