Experiment set24S402 for Pseudomonas fluorescens FW300-N2E2
Ying_Others16 rep C; time point 1
Group: carbon sourceMedia: SDM_noCarbon + 1X Ying_Others16
Culturing: pseudo6_N2E2_ML5b, tube, Aerobic, at 28 (C), shaken=180 rpm
By: Ying and Valentine on 1-Sep-23
Media components: 1.5 g/L Ammonium chloride, 0.6 g/L Potassium phosphate monobasic, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Ying_Others16 1x includes: 160 uM Gamma-Aminobutyric Acid Hydrochloride, 160 uM Ectoine, 160 uM Betaine, 160 uM N-Acetyl-L-Glutamic Acid, 160 uM Nicotinamide, 160 uM L-Ornithine, 160 uM Shikimic Acid, 160 uM spermidine, 160 uM Taurine, 160 uM Trigonelline HCl, 160 uM L-Carnitine hydrochloride, 160 uM Choline, 160 uM N-alpha-Acetyl-L-glutamate, 160 uM n-Acetyl-lysine, 160 uM n-Acetyl-muramic acid, 160 uM sn-glycero-3-phosphocholine
Specific Phenotypes
For 5 genes in this experiment
For carbon source Ying_Others16 in Pseudomonas fluorescens FW300-N2E2
For carbon source Ying_Others16 across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycine, serine and threonine metabolism
- Cyanoamino acid metabolism
- Starch and sucrose metabolism
- One carbon pool by folate
- Methane metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| glycine biosynthesis I | 1 | 1 | 1 |
| glycogen degradation I | 8 | 6 | 3 |
| dTMP de novo biosynthesis (mitochondrial) | 3 | 3 | 1 |
| glycine degradation | 3 | 3 | 1 |
| glycogen degradation II | 6 | 5 | 2 |
| superpathway of L-serine and glycine biosynthesis I | 4 | 4 | 1 |
| glycine betaine degradation I | 8 | 6 | 2 |
| starch degradation V | 4 | 3 | 1 |
| starch degradation III | 4 | 2 | 1 |
| creatinine degradation I | 4 | 1 | 1 |
| glycine betaine degradation II (mammalian) | 4 | 1 | 1 |
| folate polyglutamylation | 5 | 4 | 1 |
| creatinine degradation II | 5 | 1 | 1 |
| folate transformations II (plants) | 11 | 10 | 2 |
| glycine betaine degradation III | 7 | 7 | 1 |
| sucrose biosynthesis II | 8 | 6 | 1 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| photorespiration I | 9 | 5 | 1 |
| photorespiration III | 9 | 5 | 1 |
| photorespiration II | 10 | 6 | 1 |
| folate transformations I | 13 | 9 | 1 |
| formaldehyde assimilation I (serine pathway) | 13 | 6 | 1 |
| purine nucleobases degradation II (anaerobic) | 24 | 16 | 1 |