Experiment set23IT087 for Bacteroides thetaiotaomicron VPI-5482

NoPhage

Group: control
Media: BHIS + NophageControl + CaCl2, MgSO4 (10 mM) + Erythromycin (0.01 mg/mL), pH=7
Culturing: Btheta_ML6a, 48-well_plate, Anaerobic, at 30 (C)
By: VM on 7/27/21
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin

Specific Phenotypes

For 2 genes in this experiment

For control NophageControl in Bacteroides thetaiotaomicron VPI-5482

For control NophageControl across organisms

SEED Subsystems

Subsystem	#Specific
Biotin biosynthesis	1
D-Galacturonate and D-Glucuronate Utilization	1
ECF class transporters	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose and glucuronate interconversions

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
4-deoxy-L-threo-hex-4-enopyranuronate degradation	5	5	1
superpathway of microbial D-galacturonate and D-glucuronate degradation	31	15	1