Experiment set23IT061 for Bacteroides thetaiotaomicron VPI-5482
DAC20_cps8_0.1MOI
Group: phageMedia: BHIS + phage_DAC20 (0.1 MOI) + CaCl2, MgSO4 (10 mM) + Erythromycin (0.01 mg/mL), pH=7
Culturing: Btheta_ML6a, 48-well_plate, Anaerobic, at 30 (C)
By: VM on 7/27/21
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin
Specific Phenotypes
For 3 genes in this experiment
For phage phage_DAC20 in Bacteroides thetaiotaomicron VPI-5482
For phage phage_DAC20 across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycolysis / Gluconeogenesis
- Fructose and mannose metabolism
- Galactose metabolism
- Starch and sucrose metabolism
- Nucleotide sugars metabolism
- Streptomycin biosynthesis
- Aminosugars metabolism
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| trehalose degradation I (low osmolarity) | 2 | 1 | 1 |
| trehalose degradation II (cytosolic) | 2 | 1 | 1 |
| trehalose degradation V | 3 | 2 | 1 |
| GDP-α-D-glucose biosynthesis | 3 | 2 | 1 |
| trehalose degradation IV | 3 | 2 | 1 |
| sucrose degradation III (sucrose invertase) | 4 | 3 | 1 |
| glucose and glucose-1-phosphate degradation | 5 | 3 | 1 |
| glycogen degradation II | 6 | 4 | 1 |
| UDP-N-acetyl-D-glucosamine biosynthesis II | 6 | 3 | 1 |
| UDP-N-acetyl-D-galactosamine biosynthesis II | 7 | 4 | 1 |
| glycogen degradation I | 8 | 7 | 1 |
| sucrose biosynthesis II | 8 | 5 | 1 |
| 1,3-propanediol biosynthesis (engineered) | 9 | 6 | 1 |
| chitin biosynthesis | 9 | 4 | 1 |
| glycolysis III (from glucose) | 11 | 11 | 1 |
| homolactic fermentation | 12 | 11 | 1 |
| Bifidobacterium shunt | 15 | 12 | 1 |
| heterolactic fermentation | 18 | 14 | 1 |