Experiment set23IT029 for Klebsiella michiganensis M5al

Group: supernatant control
Media: Vogels_fungal_media (0.1x)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Cat_Manuel_Trenton on 3-Mar-21
Media components: 0.25 g/L Trisodium citrate dihydrate, 0.5 g/L Potassium phosphate monobasic, 0.2 g/L Ammonium Nitrate, 0.02 g/L Magnesium Sulfate Heptahydrate, 0.01 g/L Calcium chloride dihydrate, 0.025 g/L Citric Acid, 0.025 g/L Zinc sulfate heptahydrate, 0.005 g/L ammonium iron(II) sulfate hexahydrate, 0.00125 g/L Copper (II) sulfate pentahydrate, 0.00025 g/L Manganese (II) sulfate monohydrate, 0.00025 g/L Boric Acid, 0.00025 g/L Sodium Molybdate Dihydrate, 2.5e-05 g/L biotin, 1.5 g/L D-Glucose (final concentrations)

Subsystem	#Specific
DNA-replication	1
DNA Repair Base Excision	1
DNA repair, bacterial RecFOR pathway	1
Methionine Biosynthesis	1

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
S-adenosyl-L-methionine salvage II	3	3	1
S-adenosyl-L-methionine salvage I	4	4	1
L-methionine biosynthesis III	4	2	1
L-methionine biosynthesis I	5	5	1
seleno-amino acid biosynthesis (plants)	5	3	1
L-methionine biosynthesis II	6	5	1
superpathway of L-homoserine and L-methionine biosynthesis	8	8	1
superpathway of L-methionine biosynthesis (transsulfuration)	9	9	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	9	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	10	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	1
superpathway of seleno-compound metabolism	19	6	1
aspartate superpathway	25	24	1