Experiment set23IT014 for Klebsiella michiganensis M5al

Compare to:

Vogels_fungal_media; pH 5.5

Group: supernatant control
Media: Vogels_fungal_media, pH=5.5
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Cat_Manuel_Trenton on 3-Mar-21
Media components: 2.5 g/L Trisodium citrate dihydrate, 5 g/L Potassium phosphate monobasic, 2 g/L Ammonium Nitrate, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.1 g/L Calcium chloride dihydrate, 0.25 g/L Citric Acid, 0.25 g/L Zinc sulfate heptahydrate, 0.05 g/L ammonium iron(II) sulfate hexahydrate, 0.0125 g/L Copper (II) sulfate pentahydrate, 0.0025 g/L Manganese (II) sulfate monohydrate, 0.0025 g/L Boric Acid, 0.0025 g/L Sodium Molybdate Dihydrate, 0.00025 g/L biotin, 15 g/L D-Glucose

Specific Phenotypes

For 2 genes in this experiment

SEED Subsystems

Subsystem #Specific
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. 1
Folate Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamine degradation I 1 1 1
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I 2 2 1
L-glutamate biosynthesis I 2 2 1
4-aminobenzoate biosynthesis I 2 2 1
ammonia assimilation cycle III 3 3 1
L-asparagine biosynthesis III (tRNA-dependent) 4 3 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 3 1
L-glutamate and L-glutamine biosynthesis 7 6 1
superpathway of thiamine diphosphate biosynthesis III (eukaryotes) 7 3 1
L-citrulline biosynthesis 8 6 1
chloramphenicol biosynthesis 9 1 1
superpathway of thiamine diphosphate biosynthesis I 10 10 1
superpathway of tetrahydrofolate biosynthesis 10 9 1
superpathway of thiamine diphosphate biosynthesis II 11 9 1
superpathway of candicidin biosynthesis 11 3 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 11 1
superpathway of L-citrulline metabolism 12 8 1
superpathway of chorismate metabolism 59 54 1