Experiment set22IT077 for Pseudomonas fluorescens SBW25-INTG

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D-Glucosamine Hydrochloride 10 mM carbon source

Group: carbon source
Media: MME_noNitrogen_noCarbon + D-Glucosamine Hydrochloride (10 mM) + Ammonium chloride (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 8-Mar-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 12 genes in this experiment

For carbon source D-Glucosamine Hydrochloride in Pseudomonas fluorescens SBW25-INTG

For carbon source D-Glucosamine Hydrochloride across organisms

SEED Subsystems

Subsystem #Specific
Chitin and N-acetylglucosamine utilization 2
D-gluconate and ketogluconates metabolism 1
NAD and NADP cofactor biosynthesis global 1
Ribosomal protein S12p Asp methylthiotransferase 1
Sialic Acid Metabolism 1
UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
N-acetylglucosamine degradation I 2 2 1
L-tryptophan degradation I (via anthranilate) 3 3 1
N-acetylglucosamine degradation II 3 2 1
D-galactosamine and N-acetyl-D-galactosamine degradation 4 2 1
L-tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde 5 3 1
3-hydroxy-4-methyl-anthranilate biosynthesis II 5 3 1
N-acetyl-D-galactosamine degradation 5 2 1
superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation 6 2 1
chitin derivatives degradation 8 2 1
NAD de novo biosynthesis II (from tryptophan) 9 6 1
L-tryptophan degradation XI (mammalian, via kynurenine) 23 7 2
L-tryptophan degradation IX 12 4 1
L-tryptophan degradation XII (Geobacillus) 12 4 1
peptidoglycan recycling I 14 11 1
superpathway of NAD biosynthesis in eukaryotes 14 9 1
L-tryptophan degradation III (eukaryotic) 15 6 1
superpathway of N-acetylneuraminate degradation 22 14 1
superpathway of aromatic compound degradation via 3-oxoadipate 35 19 1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate 42 13 1