Experiment set22IT071 for Pseudomonas fluorescens SBW25-INTG
caffeic acid 2 mM carbon source
Group: carbon sourceMedia: MME_noNitrogen_noCarbon + caffeic acid (2 mM) + Ammonium chloride (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Joshua Elmore on 8-Mar-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 9 genes in this experiment
For carbon source caffeic acid in Pseudomonas fluorescens SBW25-INTG
For carbon source caffeic acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Oxidative stress | 1 |
| Phenylpropanoid compound degradation | 1 |
| Photorespiration (oxidative C2 cycle) | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| superoxide radicals degradation | 2 | 2 | 1 |
| methanol oxidation to formaldehyde IV | 2 | 1 | 1 |
| ethanol degradation IV | 3 | 3 | 1 |
| reactive oxygen species degradation | 4 | 4 | 1 |