Experiment set22IT061 for Pseudomonas fluorescens SBW25-INTG
Betaine hydrochloride 10 mM carbon source
Group: carbon sourceMedia: MME_noNitrogen_noCarbon + Betaine hydrochloride (10 mM) + Ammonium chloride (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 8-Mar-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 6 genes in this experiment
For carbon source Betaine hydrochloride in Pseudomonas fluorescens SBW25-INTG
For carbon source Betaine hydrochloride across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Methane metabolism
- Nicotinate and nicotinamide metabolism
- Folate biosynthesis
- Biosynthesis of phenylpropanoids
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamine degradation I | 1 | 1 | 1 |
| formaldehyde oxidation IV (thiol-independent) | 1 | 1 | 1 |
| 4-aminobenzoate biosynthesis I | 2 | 2 | 1 |
| L-glutamate biosynthesis I | 2 | 2 | 1 |
| ammonia assimilation cycle III | 3 | 3 | 1 |
| L-asparagine biosynthesis III (tRNA-dependent) | 4 | 4 | 1 |
| glutaminyl-tRNAgln biosynthesis via transamidation | 4 | 3 | 1 |
| NAD(P)/NADPH interconversion | 6 | 3 | 1 |
| methanol oxidation to carbon dioxide | 6 | 3 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 6 | 1 |
| L-citrulline biosynthesis | 8 | 8 | 1 |
| chloramphenicol biosynthesis | 9 | 1 | 1 |
| superpathway of tetrahydrofolate biosynthesis | 10 | 8 | 1 |
| superpathway of candicidin biosynthesis | 11 | 4 | 1 |
| superpathway of L-citrulline metabolism | 12 | 10 | 1 |
| superpathway of tetrahydrofolate biosynthesis and salvage | 12 | 10 | 1 |
| superpathway of C1 compounds oxidation to CO2 | 12 | 5 | 1 |
| superpathway of chorismate metabolism | 59 | 42 | 1 |