Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
| Pathway | #Steps | #Present | #Specific |
|---|
| 2-oxoisovalerate decarboxylation to isobutanoyl-CoA | 3 | 3 | 3 |
| L-asparagine degradation I | 1 | 1 | 1 |
| glycine biosynthesis IV | 1 | 1 | 1 |
| L-methionine degradation II | 3 | 3 | 2 |
| putrescine degradation V | 2 | 2 | 1 |
| L-lactaldehyde degradation (aerobic) | 2 | 2 | 1 |
| L-threonine degradation I | 6 | 4 | 3 |
| L-threonine degradation IV | 2 | 1 | 1 |
| putrescine degradation I | 2 | 1 | 1 |
| ethylene glycol degradation | 2 | 1 | 1 |
| L-threonine degradation V | 2 | 1 | 1 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 3 |
| 2-methylcitrate cycle I | 5 | 5 | 2 |
| L-homoserine biosynthesis | 3 | 3 | 1 |
| ethanol degradation IV | 3 | 3 | 1 |
| 2-oxoglutarate decarboxylation to succinyl-CoA | 3 | 3 | 1 |
| glycine cleavage | 3 | 3 | 1 |
| pyruvate decarboxylation to acetyl CoA I | 3 | 3 | 1 |
| benzoyl-CoA biosynthesis | 3 | 3 | 1 |
| glycine biosynthesis II | 3 | 3 | 1 |
| ethanol degradation II | 3 | 3 | 1 |
| glyoxylate cycle | 6 | 5 | 2 |
| 2-methylcitrate cycle II | 6 | 5 | 2 |
| hypotaurine degradation | 3 | 2 | 1 |
| ethanol degradation III | 3 | 2 | 1 |
| L-asparagine degradation III (mammalian) | 3 | 2 | 1 |
| methylglyoxal degradation V | 3 | 2 | 1 |
| methylglyoxal degradation IV | 3 | 2 | 1 |
| putrescine degradation IV | 3 | 2 | 1 |
| histamine degradation | 3 | 1 | 1 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 4 |
| partial TCA cycle (obligate autotrophs) | 8 | 8 | 2 |
| nitrogen remobilization from senescing leaves | 8 | 6 | 2 |
| superpathway of L-aspartate and L-asparagine biosynthesis | 4 | 3 | 1 |
| putrescine degradation III | 4 | 3 | 1 |
| phytol degradation | 4 | 3 | 1 |
| D-arabinose degradation II | 4 | 2 | 1 |
| L-tryptophan degradation X (mammalian, via tryptamine) | 4 | 2 | 1 |
| fatty acid α-oxidation I (plants) | 4 | 2 | 1 |
| TCA cycle V (2-oxoglutarate synthase) | 9 | 7 | 2 |
| TCA cycle II (plants and fungi) | 9 | 7 | 2 |
| TCA cycle IV (2-oxoglutarate decarboxylase) | 9 | 7 | 2 |
| TCA cycle VI (Helicobacter) | 9 | 7 | 2 |
| TCA cycle VII (acetate-producers) | 9 | 7 | 2 |
| superpathway of L-threonine metabolism | 18 | 11 | 4 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 12 | 3 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 11 | 3 |
| hypoglycin biosynthesis | 14 | 4 | 3 |
| adipate degradation | 5 | 5 | 1 |
| TCA cycle I (prokaryotic) | 10 | 9 | 2 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 4 | 1 |
| adipate biosynthesis | 5 | 4 | 1 |
| octane oxidation | 5 | 4 | 1 |
| propanoyl-CoA degradation II | 5 | 4 | 1 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 4 | 1 |
| mitochondrial NADPH production (yeast) | 5 | 4 | 1 |
| TCA cycle III (animals) | 10 | 7 | 2 |
| lactate biosynthesis (archaea) | 5 | 3 | 1 |
| acrylate degradation I | 5 | 3 | 1 |
| sphingosine and sphingosine-1-phosphate metabolism | 10 | 4 | 2 |
| dopamine degradation | 5 | 2 | 1 |
| ferrichrome A biosynthesis | 5 | 2 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 2 | 1 |
| N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation | 5 | 2 | 1 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 2 |
| reductive TCA cycle I | 11 | 6 | 2 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 3 |
| superpathway of L-threonine biosynthesis | 6 | 6 | 1 |
| superpathway of glyoxylate bypass and TCA | 12 | 11 | 2 |
| L-leucine degradation I | 6 | 5 | 1 |
| β-alanine biosynthesis II | 6 | 5 | 1 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 1 |
| methyl ketone biosynthesis (engineered) | 6 | 4 | 1 |
| 3-methyl-branched fatty acid α-oxidation | 6 | 3 | 1 |
| reductive TCA cycle II | 12 | 5 | 2 |
| alkane oxidation | 6 | 1 | 1 |
| noradrenaline and adrenaline degradation | 13 | 8 | 2 |
| fatty acid β-oxidation I (generic) | 7 | 6 | 1 |
| superpathway of glycol metabolism and degradation | 7 | 5 | 1 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 5 | 1 |
| serotonin degradation | 7 | 4 | 1 |
| benzoyl-CoA degradation I (aerobic) | 7 | 3 | 1 |
| ceramide degradation by α-oxidation | 7 | 2 | 1 |
| limonene degradation IV (anaerobic) | 7 | 1 | 1 |
| superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle | 22 | 18 | 3 |
| superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) | 8 | 7 | 1 |
| superpathway of L-homoserine and L-methionine biosynthesis | 8 | 6 | 1 |
| L-valine degradation I | 8 | 6 | 1 |
| mixed acid fermentation | 16 | 11 | 2 |
| superpathway of methylglyoxal degradation | 8 | 5 | 1 |
| L-rhamnose degradation II | 8 | 4 | 1 |
| superpathway of ornithine degradation | 8 | 4 | 1 |
| aromatic biogenic amine degradation (bacteria) | 8 | 4 | 1 |
| ceramide and sphingolipid recycling and degradation (yeast) | 16 | 4 | 2 |
| superpathway of L-methionine biosynthesis (transsulfuration) | 9 | 7 | 1 |
| superpathway of S-adenosyl-L-methionine biosynthesis | 9 | 7 | 1 |
| valproate β-oxidation | 9 | 6 | 1 |
| Entner-Doudoroff pathway II (non-phosphorylative) | 9 | 6 | 1 |
| phenylacetate degradation I (aerobic) | 9 | 4 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 1 |
| methylaspartate cycle | 19 | 9 | 2 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 9 | 1 |
| 3-phenylpropanoate degradation | 10 | 5 | 1 |
| superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation | 11 | 7 | 1 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 1 |
| superpathway of phenylethylamine degradation | 11 | 6 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 4 | 2 |
| oleate β-oxidation | 35 | 33 | 3 |
| superpathway of L-methionine biosynthesis (by sulfhydrylation) | 12 | 12 | 1 |
| superpathway of fucose and rhamnose degradation | 12 | 5 | 1 |
| ethene biosynthesis V (engineered) | 25 | 18 | 2 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 22 | 2 |
| superpathway of L-arginine and L-ornithine degradation | 13 | 9 | 1 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 3 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 3 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis II | 15 | 13 | 1 |
| cyclosporin A biosynthesis | 15 | 2 | 1 |
| superpathway of L-lysine, L-threonine and L-methionine biosynthesis I | 18 | 16 | 1 |
| aspartate superpathway | 25 | 22 | 1 |
| platensimycin biosynthesis | 26 | 6 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |
| superpathway of pentose and pentitol degradation | 42 | 16 | 1 |