Experiment set22IT041 for Pseudomonas fluorescens SBW25-INTG

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L-Ornithine 10 mM carbon source

Group: carbon source
Media: MME_noNitrogen_noCarbon + L-Ornithine (10 mM) + Ammonium chloride (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 8-Mar-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 50 genes in this experiment

For carbon source L-Ornithine in Pseudomonas fluorescens SBW25-INTG

For carbon source L-Ornithine across organisms

SEED Subsystems

Subsystem #Specific
Arginine and Ornithine Degradation 12
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 8
Methionine Biosynthesis 4
Cysteine Biosynthesis 3
Polyamine Metabolism 3
Conserved gene cluster possibly involved in RNA metabolism 2
Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate 2
Acid resistance mechanisms 1
Arginine Deiminase Pathway 1
Entner-Doudoroff Pathway 1
Ethanolamine utilization 1
Fermentations: Lactate 1
Fermentations: Mixed acid 1
Fructose utilization 1
Glutamate dehydrogenases 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria 1
MLST 1
Methionine Salvage 1
Methylglyoxal Metabolism 1
Proline, 4-hydroxyproline uptake and utilization 1
Propanediol utilization 1
Respiratory dehydrogenases 1 1
Terminal cytochrome C oxidases 1
Thiamin biosynthesis 1
Threonine anaerobic catabolism gene cluster 1
Threonine and Homoserine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) 3 3 3
putrescine biosynthesis II 3 3 3
L-cysteine biosynthesis I 2 2 2
L-ornithine degradation I (L-proline biosynthesis) 1 1 1
arginine dependent acid resistance 1 1 1
L-glutamate degradation I 1 1 1
L-arginine degradation I (arginase pathway) 3 3 2
L-proline degradation I 3 3 2
glycine betaine biosynthesis IV (from glycine) 3 2 2
4-aminobutanoate degradation III 2 2 1
L-arginine degradation VII (arginase 3 pathway) 2 2 1
indole-3-acetate biosynthesis IV (bacteria) 2 2 1
L-lactaldehyde degradation (aerobic) 2 2 1
acrylonitrile degradation I 2 2 1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway) 2 2 1
acetate and ATP formation from acetyl-CoA I 2 2 1
indole-3-acetate biosynthesis III (bacteria) 2 2 1
putrescine degradation V 2 2 1
putrescine biosynthesis I 2 2 1
putrescine degradation I 2 1 1
ethylene glycol degradation 2 1 1
L-proline biosynthesis II (from arginine) 2 1 1
arsenite to oxygen electron transfer 2 1 1
sulfoacetaldehyde degradation I 2 1 1
L-arginine degradation II (AST pathway) 5 5 2
seleno-amino acid biosynthesis (plants) 5 3 2
superpathway of polyamine biosynthesis II 8 6 3
L-alanine degradation II (to D-lactate) 3 3 1
superpathway of acetate utilization and formation 3 3 1
L-homoserine biosynthesis 3 3 1
ethanol degradation II 3 3 1
L-proline biosynthesis III (from L-ornithine) 3 3 1
pyruvate fermentation to acetate II 3 3 1
ethanol degradation IV 3 3 1
L-ornithine biosynthesis II 3 3 1
pyruvate fermentation to acetate VII 3 2 1
putrescine degradation IV 3 2 1
L-arginine degradation X (arginine monooxygenase pathway) 3 2 1
methylglyoxal degradation V 3 2 1
pyruvate fermentation to acetate I 3 2 1
pyruvate fermentation to acetate IV 3 2 1
ethanol degradation III 3 2 1
superpathway of acrylonitrile degradation 3 2 1
methylglyoxal degradation IV 3 2 1
hypotaurine degradation 3 2 1
5'-deoxyadenosine degradation I 3 1 1
arsenite to oxygen electron transfer (via azurin) 3 1 1
ethene biosynthesis IV (engineered) 3 1 1
histamine degradation 3 1 1
L-Nδ-acetylornithine biosynthesis 7 6 2
L-citrulline biosynthesis 8 8 2
superpathway of putrescine biosynthesis 4 4 1
L-arginine degradation VI (arginase 2 pathway) 4 4 1
phytol degradation 4 3 1
putrescine degradation III 4 3 1
aerobic respiration II (cytochrome c) (yeast) 4 3 1
aerobic respiration I (cytochrome c) 4 3 1
pyruvate fermentation to acetate and (S)-lactate I 4 3 1
L-cysteine biosynthesis VII (from S-sulfo-L-cysteine) 4 3 1
pyruvate fermentation to acetate and lactate II 4 2 1
D-arabinose degradation II 4 2 1
L-tryptophan degradation X (mammalian, via tryptamine) 4 2 1
fatty acid α-oxidation I (plants) 4 2 1
spermidine biosynthesis III 4 1 1
ethene biosynthesis II (microbes) 4 1 1
sulfolactate degradation II 4 1 1
5'-deoxyadenosine degradation II 4 1 1
N-3-oxalyl-L-2,3-diaminopropanoate biosynthesis 4 1 1
superpathway of sulfate assimilation and cysteine biosynthesis 9 9 2
L-ornithine biosynthesis I 5 5 1
L-lysine degradation IV 5 5 1
L-arginine degradation XIII (reductive Stickland reaction) 5 5 1
octane oxidation 5 4 1
ethanolamine utilization 5 4 1
mitochondrial NADPH production (yeast) 5 4 1
lactate biosynthesis (archaea) 5 3 1
acetylene degradation (anaerobic) 5 3 1
sphingosine and sphingosine-1-phosphate metabolism 10 4 2
dopamine degradation 5 2 1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation II 5 2 1
(S)-propane-1,2-diol degradation 5 2 1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation III 5 2 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 7 2
superpathway of L-threonine biosynthesis 6 6 1
superpathway of L-citrulline metabolism 12 10 2
L-lysine degradation X 6 5 1
hydrogen sulfide biosynthesis II (mammalian) 6 4 1
L-threonine degradation I 6 4 1
thiazole component of thiamine diphosphate biosynthesis I 6 4 1
Fe(II) oxidation 6 3 1
3-methyl-branched fatty acid α-oxidation 6 3 1
superpathway of sulfolactate degradation 6 2 1
methanogenesis from acetate 6 2 1
L-lysine degradation III 6 2 1
superpathway of taurine degradation 6 2 1
D-cycloserine biosynthesis 6 1 1
(5R)-carbapenem carboxylate biosynthesis 6 1 1
alkane oxidation 6 1 1
superpathway of L-arginine and L-ornithine degradation 13 9 2
noradrenaline and adrenaline degradation 13 8 2
superpathway of glycol metabolism and degradation 7 5 1
thiazole component of thiamine diphosphate biosynthesis II 7 5 1
serotonin degradation 7 4 1
L-lysine degradation I 7 4 1
4-aminobutanoate degradation V 7 3 1
L-glutamate degradation XI (reductive Stickland reaction) 7 3 1
L-cysteine biosynthesis VI (reverse transsulfuration) 7 3 1
acetyl-CoA fermentation to butanoate 7 3 1
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I 7 2 1
ceramide degradation by α-oxidation 7 2 1
limonene degradation IV (anaerobic) 7 1 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 7 1
superpathway of L-homoserine and L-methionine biosynthesis 8 6 1
superpathway of polyamine biosynthesis I 8 6 1
superpathway of methylglyoxal degradation 8 5 1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) 8 4 1
L-rhamnose degradation II 8 4 1
aromatic biogenic amine degradation (bacteria) 8 4 1
superpathway of ornithine degradation 8 4 1
ceramide and sphingolipid recycling and degradation (yeast) 16 4 2
superpathway of arginine and polyamine biosynthesis 17 15 2
L-arginine biosynthesis I (via L-ornithine) 9 9 1
L-lysine biosynthesis I 9 9 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 2
L-arginine biosynthesis III (via N-acetyl-L-citrulline) 9 8 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 7 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
Entner-Doudoroff pathway II (non-phosphorylative) 9 6 1
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 5 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 4 1
superpathway of L-alanine fermentation (Stickland reaction) 9 4 1
superpathway of seleno-compound metabolism 19 8 2
L-arginine biosynthesis II (acetyl cycle) 10 10 1
superpathway of thiamine diphosphate biosynthesis I 10 8 1
L-glutamate degradation V (via hydroxyglutarate) 10 6 1
L-lysine fermentation to acetate and butanoate 10 3 1
superpathway of thiamine diphosphate biosynthesis II 11 9 1
L-methionine salvage cycle III 11 5 1
gallate degradation III (anaerobic) 11 3 1
L-methionine salvage cycle II (plants) 11 3 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 12 1
superpathway of fucose and rhamnose degradation 12 5 1
indole-3-acetate biosynthesis II 12 5 1
L-methionine salvage cycle I (bacteria and plants) 12 4 1
aspartate superpathway 25 22 2
superpathway of L-isoleucine biosynthesis I 13 13 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 4 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
mixed acid fermentation 16 11 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 7 1
heterolactic fermentation 18 13 1
superpathway of L-threonine metabolism 18 11 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 13 1
methylaspartate cycle 19 9 1
superpathway of methanogenesis 21 2 1
superpathway of L-lysine degradation 43 17 2
superpathway of N-acetylneuraminate degradation 22 14 1
purine nucleobases degradation II (anaerobic) 24 16 1
superpathway of pentose and pentitol degradation 42 16 1