Experiment set22IT027 for Pseudomonas fluorescens FW300-N2E2

Compare to:

nitrogen source Ammonium chloride 20mM

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_4187 -7.1 -10.7 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_3661 -6.4 -11.2 hypothetical protein compare
Pf6N2E2_3783 -6.1 -7.3 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_64 -6.1 -5.3 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_3264 -6.1 -6.1 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_5291 -5.9 -27.3 Fumarylacetoacetase (EC 3.7.1.2) compare
Pf6N2E2_2320 -5.8 -14.1 Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein compare
Pf6N2E2_2823 -5.6 -3.9 Multidrug efflux RND membrane fusion protein MexC compare
Pf6N2E2_2318 -5.4 -28.5 Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_2574 -5.2 -3.3 Flagellar synthesis regulator FleN compare
Pf6N2E2_4279 -5.2 -16.9 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_4278 -5.0 -13.0 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_3751 -5.0 -14.7 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_3752 -4.9 -23.5 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_5177 -4.8 -13.8 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_5316 -4.8 -4.6 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_4803 -4.8 -2.7 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_4825 -4.7 -11.4 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_5069 -4.7 -7.5 putative Cytochrome bd2, subunit II compare
Pf6N2E2_66 -4.7 -12.8 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_71 -4.7 -7.9 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_3782 -4.6 -8.6 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_5337 -4.5 -27.9 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_2885 -4.5 -6.8 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
Pf6N2E2_5339 -4.4 -23.2 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_5338 -4.4 -27.8 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_5242 -4.4 -3.8 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_3841 -4.4 -3.0 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_3253 -4.3 -7.7 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_4597 -4.2 -9.9 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3786 -4.2 -8.8 Twin-arginine translocation protein TatC compare
Pf6N2E2_5068 -4.2 -13.5 putative Cytochrome bd2, subunit I compare
Pf6N2E2_4638 -4.2 -2.9 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_2825 -4.2 -6.4 RND efflux system, outer membrane lipoprotein CmeC compare
Pf6N2E2_2317 -4.2 -9.1 Putative analog of CcoH, COG3198 compare
Pf6N2E2_4048 -4.2 -7.5 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_4277 -4.1 -12.1 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_5175 -4.1 -6.8 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_5527 -4.1 -2.8 Cold shock protein CspC compare
Pf6N2E2_3630 -4.1 -8.6 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_4596 -4.0 -8.5 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_5571 -4.0 -2.2 hypothetical protein compare
Pf6N2E2_4153 -4.0 -3.9 Sensory box histidine kinase compare
Pf6N2E2_3303 -3.9 -5.9 Sensor histidine kinase PrrB (RegB) (EC 2.7.3.-) compare
Pf6N2E2_77 -3.8 -6.9 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_3839 -3.7 -5.6 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_3842 -3.7 -5.3 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_302 -3.7 -6.7 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_2258 -3.7 -11.5 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_4564 -3.6 -9.5 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_4206 -3.6 -2.0 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_5150 -3.5 -6.3 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_3984 -3.5 -15.2 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_4047 -3.5 -4.2 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_4826 -3.5 -8.5 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_2753 -3.5 -5.2 HtrA protease/chaperone protein compare
Pf6N2E2_2552 -3.5 -9.9 Cytochrome c heme lyase subunit CcmH compare
Pf6N2E2_2588 -3.4 -2.3 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_1636 -3.4 -7.9 Ferrichrome-iron receptor compare
Pf6N2E2_1776 -3.4 -1.8 Chemotaxis protein CheD compare
Pf6N2E2_3252 -3.2 -6.6 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_3932 -3.2 -3.6 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_3940 -3.2 -9.8 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_2824 -3.1 -10.3 RND efflux system, inner membrane transporter CmeB compare
Pf6N2E2_3037 -3.1 -14.6 RNA polymerase sigma factor RpoS compare
Pf6N2E2_2962 -3.0 -1.7 ABC transporter for L-Lysine, ATPase component (from data) compare
Pf6N2E2_2073 -3.0 -8.9 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_3248 -3.0 -4.8 Probable transmembrane protein compare
Pf6N2E2_2465 -3.0 -7.9 Cys regulon transcriptional activator CysB compare
Pf6N2E2_5520 -3.0 -8.6 Sensory box histidine kinase compare
Pf6N2E2_5176 -2.8 -6.2 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_2189 -2.8 -7.3 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_2899 -2.8 -5.8 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) compare
Pf6N2E2_1656 -2.7 -1.5 hypothetical protein compare
Pf6N2E2_3170 -2.7 -7.1 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_3785 -2.7 -4.5 Twin-arginine translocation protein TatB compare
Pf6N2E2_2509 -2.6 -2.1 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_3658 -2.6 -12.9 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_5252 -2.5 -3.9 FIG00956396: hypothetical protein compare
Pf6N2E2_2864 -2.5 -3.2 Gluconokinase (EC 2.7.1.12) compare
Pf6N2E2_4584 -2.5 -6.2 Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1) compare
Pf6N2E2_5494 -2.5 -5.9 Pyruvate kinase (EC 2.7.1.40) compare
Pf6N2E2_3778 -2.5 -3.8 FIG00954300: hypothetical protein compare
Pf6N2E2_3373 -2.5 -2.9 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf6N2E2_2013 -2.5 -0.8 hypothetical protein compare
Pf6N2E2_1534 -2.4 -1.1 Probable transmembrane protein compare
Pf6N2E2_4276 -2.4 -6.3 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_2506 -2.4 -14.0 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_3151 -2.4 -6.9 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_5178 -2.4 -4.9 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_2074 -2.3 -0.9 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_6082 -2.3 -3.7 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_5704 -2.2 -6.4 Transcriptional regulatory protein PhoP compare
Pf6N2E2_2510 -2.2 -3.4 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_2646 -2.2 -1.2 FIG00953416: hypothetical protein compare
Pf6N2E2_3664 -2.2 -8.8 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_2863 -2.2 -8.3 Gluconate permease compare
Pf6N2E2_2316 -2.2 -7.0 Type cbb3 cytochrome oxidase biogenesis protein CcoG, involved in Cu oxidation compare
Pf6N2E2_817 -2.2 -9.2 Protein-glutamate methylesterase (EC 3.1.1.61) compare
Pf6N2E2_63 -2.1 -3.0 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_2967 -2.1 -1.3 Cold shock protein CspA compare
Pf6N2E2_2897 -2.1 -9.7 Glucokinase (EC 2.7.1.2) compare
Pf6N2E2_2282 -2.1 -3.7 Predicted transcriptional regulator for fatty acid degradation FadQ, TetR family compare
Pf6N2E2_3328 -2.1 -1.6 transcriptional regulator compare
Pf6N2E2_5780 -2.1 -5.5 Periplasmic protease compare
Pf6N2E2_3834 -2.0 -6.9 Lipoprotein NlpD compare
Pf6N2E2_2384 -2.0 -4.0 CmpX compare
Pf6N2E2_3659 -2.0 -8.7 Protein fixF compare
Pf6N2E2_4624 -2.0 -0.9 Phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.-) compare
Pf6N2E2_5560 -2.0 -1.9 Sigma-54 dependent transcriptional regulator/sensory box protein compare
Pf6N2E2_3617 -2.0 -6.8 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) compare
Pf6N2E2_3665 -2.0 -1.3 serine/threonine protein kinase compare
Pf6N2E2_3835 -1.9 -4.7 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_5160 -1.9 -2.5 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_50 -1.9 -6.8 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_2201 -1.9 -5.6 Transmembrane regulator protein PrtR compare
Pf6N2E2_4889 -1.9 -7.7 Putative transport protein compare
Pf6N2E2_3763 -1.9 -1.5 hypothetical protein compare
Pf6N2E2_5579 -1.9 -6.0 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_1792 -1.9 -1.8 Hydroxypyruvate isomerase (EC 5.3.1.22) compare
Pf6N2E2_2938 -1.9 -5.7 Outer membrane lipoprotein compare
Pf6N2E2_4273 -1.9 -8.1 ATP-dependent DNA helicase UvrD/PcrA compare
Pf6N2E2_4743 -1.9 -0.6 hypothetical protein compare
Pf6N2E2_5219 -1.9 -6.5 FIG140336: TPR domain protein compare
Pf6N2E2_2218 -1.9 -4.2 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_75 -1.8 -6.7 Colicin V production protein compare
Pf6N2E2_2292 -1.8 -4.9 Universal stress protein family COG0589 compare
Pf6N2E2_3610 -1.8 -3.2 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_2707 -1.8 -4.2 Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) compare
Pf6N2E2_6164 -1.8 -1.8 tRNA-Val-TAC compare
Pf6N2E2_3300 -1.8 -3.1 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_2447 -1.8 -3.7 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_4014 -1.8 -7.7 Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16) compare
Pf6N2E2_6063 -1.8 -8.7 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79) compare
Pf6N2E2_3134 -1.8 -1.3 hypothetical protein compare
Pf6N2E2_2518 -1.7 -2.9 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_2081 -1.7 -2.1 putative exported protein compare
Pf6N2E2_659 -1.7 -1.5 Transcriptional regulatory protein ompR compare
Pf6N2E2_3587 -1.7 -2.8 Azurin compare
Pf6N2E2_5728 -1.7 -4.4 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_4369 -1.7 -1.6 Exodeoxyribonuclease III (EC 3.1.11.2) compare
Pf6N2E2_4394 -1.7 -4.1 Putrescine utilization regulator compare
Pf6N2E2_89 -1.7 -1.6 hypothetical protein compare
Pf6N2E2_4585 -1.7 -9.6 Cell division protein FtsX compare
Pf6N2E2_5666 -1.7 -7.6 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_5703 -1.7 -5.3 outer membrane protein H1 compare
Pf6N2E2_3198 -1.7 -1.6 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_2513 -1.7 -3.8 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_5226 -1.7 -0.8 FIG00955589: hypothetical protein compare
Pf6N2E2_5727 -1.7 -4.1 FIG00955360: hypothetical protein compare
Pf6N2E2_3969 -1.7 -0.7 Membrane-fusion protein compare
Pf6N2E2_292 -1.7 -7.7 ATP-dependent Clp protease ATP-binding subunit ClpA compare
Pf6N2E2_2309 -1.7 -3.3 hypothetical protein compare
Pf6N2E2_2706 -1.6 -3.1 Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54) compare
Pf6N2E2_3534 -1.6 -1.1 Cobalamin biosynthesis protein CobG compare
Pf6N2E2_5936 -1.6 -1.0 Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48) compare
Pf6N2E2_4025 -1.6 -0.7 hypothetical protein compare
Pf6N2E2_4797 -1.6 -0.7 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_2895 -1.6 -7.4 Integral membrane sensor signal transduction histidine kinase (EC 2.7.13.3), glucose catabolism cluster compare
Pf6N2E2_1606 -1.6 -4.0 Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-) compare
Pf6N2E2_2225 -1.6 -1.2 FIG00953489: hypothetical protein compare
Pf6N2E2_5156 -1.6 -1.1 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_2755 -1.6 -1.4 Sigma factor RpoE negative regulatory protein RseA compare
Pf6N2E2_1735 -1.6 -1.9 PhnB protein compare
Pf6N2E2_3260 -1.6 -2.8 Uncharacterized ABC transporter, ATP-binding protein YrbF compare
Pf6N2E2_3666 -1.5 -3.5 InaA protein compare
Pf6N2E2_5170 -1.5 -10.1 FIG00955330: hypothetical protein compare
Pf6N2E2_1916 -1.5 -2.6 Transcriptional regulator, LysR family compare
Pf6N2E2_2129 -1.5 -1.7 Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup compare
Pf6N2E2_2890 -1.5 -7.1 Glucose ABC transport system, inner membrane component 2 compare
Pf6N2E2_5524 -1.5 -8.2 Outer membrane porin, OprD family compare
Pf6N2E2_1672 -1.5 -7.0 hypothetical protein compare
Pf6N2E2_1258 -1.5 -2.0 FIG00960119: hypothetical protein compare
Pf6N2E2_3941 -1.5 -1.4 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_2827 -1.5 -0.7 hypothetical protein compare
Pf6N2E2_3505 -1.5 -0.7 Translation initiation factor SUI1-related protein compare
Pf6N2E2_6093 -1.5 -2.4 hypothetical protein compare
Pf6N2E2_4362 -1.5 -1.0 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_4372 -1.5 -2.9 FIG00460773: hypothetical protein compare
Pf6N2E2_1165 -1.5 -1.7 hypothetical protein compare
Pf6N2E2_4160 -1.4 -1.6 Cytochrome c5 compare
Pf6N2E2_2397 -1.4 -2.3 Two-component system response regulator QseB compare
Pf6N2E2_5505 -1.4 -1.4 hypothetical protein compare
Pf6N2E2_3908 -1.4 -3.5 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_2892 -1.4 -8.6 Glucose ABC transport system, periplasmic sugar-binding protein compare
Pf6N2E2_521 -1.4 -2.8 hypothetical protein compare
Pf6N2E2_96 -1.4 -4.3 FIG011065: hypothetical protein compare
Pf6N2E2_5575 -1.4 -1.0 Glycerol uptake facilitator protein compare
Pf6N2E2_3251 -1.4 -6.7 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_3663 -1.4 -8.6 Glycosyl transferase compare
Pf6N2E2_4392 -1.4 -1.6 Translation initiation inhibitor compare
Pf6N2E2_5014 -1.4 -0.4 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_2586 -1.4 -1.5 Hpt domain protein compare
Pf6N2E2_4999 -1.4 -6.0 Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-) compare
Pf6N2E2_3340 -1.4 -7.6 Fructose repressor FruR, LacI family compare
Pf6N2E2_248 -1.4 -3.3 hypothetical protein compare
Pf6N2E2_2311 -1.3 -7.4 Cytochrome c oxidase subunit CcoO (EC 1.9.3.1) compare
Pf6N2E2_3833 -1.3 -3.9 hypothetical protein compare
Pf6N2E2_4077 -1.3 -3.1 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_2810 -1.3 -2.9 Cardiolipin synthetase (EC 2.7.8.-) compare


Specific Phenotypes

For 3 genes in this experiment

For nitrogen source Ammonium chloride in Pseudomonas fluorescens FW300-N2E2

For nitrogen source Ammonium chloride across organisms