Experiment set22IT010 for Pseudomonas fluorescens SBW25-INTG

Phloretic Acid 2 mM carbon source

Group: carbon source
Media: MME_noNitrogen_noCarbon + 4-Hydroxyphenylpropionic acid (2 mM) + Ammonium chloride (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Joshua Elmore on 8-Mar-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 12 genes in this experiment

For carbon source 4-Hydroxyphenylpropionic acid in Pseudomonas fluorescens SBW25-INTG

For carbon source 4-Hydroxyphenylpropionic acid across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	2
Bacterial Chemotaxis	1
Flagellar motility	1
Two-component regulatory systems in Campylobacter	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
oxalate degradation II	3	1	1
adipate degradation	5	5	1
fatty acid salvage	6	6	1
oleate β-oxidation	35	33	3
2-methyl-branched fatty acid β-oxidation	14	11	1