Experiment set21IT077 for Pseudomonas fluorescens SBW25-INTG
Glycerol 20 mM carbon source
Group: carbon sourceMedia: MME_noNitrogen_noCarbon + Glycerol (20 mM) + Ammonium chloride (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 8-Mar-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 1 genes in this experiment
For carbon source Glycerol in Pseudomonas fluorescens SBW25-INTG
For carbon source Glycerol across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glycerol and Glycerol-3-phosphate Uptake and Utilization | 1 |
| Glycerol fermenation to 1,3-propanediol | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| arsenate detoxification III | 2 | 2 | 1 |
| glycerol degradation I | 3 | 3 | 1 |
| glycerol and glycerophosphodiester degradation | 4 | 4 | 1 |
| arsenic detoxification (plants) | 6 | 4 | 1 |
| arsenic detoxification (yeast) | 12 | 4 | 1 |
| arsenic detoxification (mammals) | 17 | 8 | 1 |