Experiment set21IT075 for Pseudomonas fluorescens SBW25-INTG
D-Sorbitol 10 mM carbon source
Group: carbon sourceMedia: MME_noNitrogen_noCarbon + D-Sorbitol (10 mM) + Ammonium chloride (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 8-Mar-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 11 genes in this experiment
For carbon source D-Sorbitol in Pseudomonas fluorescens SBW25-INTG
For carbon source D-Sorbitol across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fructose and mannose metabolism
- Nucleotide sugars metabolism
- Pentose and glucuronate interconversions
- Ascorbate and aldarate metabolism
- C21-Steroid hormone metabolism
- Glycine, serine and threonine metabolism
- Bisphenol A degradation
- Starch and sucrose metabolism
- Polyketide sugar unit biosynthesis
- Aminosugars metabolism
- Linoleic acid metabolism
- Tetrachloroethene degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Retinol metabolism
- Alkaloid biosynthesis I
- Insect hormone biosynthesis
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide products
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-sorbitol degradation I | 3 | 3 | 2 |
| xylitol degradation I | 2 | 2 | 1 |
| D-xylose degradation I | 2 | 2 | 1 |
| arsenate detoxification III | 2 | 2 | 1 |
| D-arabinitol degradation I | 2 | 1 | 1 |
| mannitol cycle | 5 | 3 | 2 |
| glycerol degradation I | 3 | 3 | 1 |
| sucrose degradation I (sucrose phosphotransferase) | 3 | 2 | 1 |
| glycerol and glycerophosphodiester degradation | 4 | 4 | 1 |
| sucrose degradation IV (sucrose phosphorylase) | 4 | 3 | 1 |
| sucrose degradation III (sucrose invertase) | 4 | 3 | 1 |
| D-altritol and galactitol degradation | 4 | 1 | 1 |
| sucrose degradation VII (sucrose 3-dehydrogenase) | 4 | 1 | 1 |
| sucrose degradation II (sucrose synthase) | 5 | 4 | 1 |
| arsenic detoxification (plants) | 6 | 4 | 1 |
| arsenic detoxification (yeast) | 12 | 4 | 1 |
| superpathway of glucose and xylose degradation | 17 | 16 | 1 |
| arsenic detoxification (mammals) | 17 | 8 | 1 |
| heterolactic fermentation | 18 | 13 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 14 | 1 |
| superpathway of pentose and pentitol degradation | 42 | 16 | 1 |