Experiment set21IT072 for Pseudomonas fluorescens SBW25-INTG
Sodium L-Lactate 20 mM carbon source
Group: carbon sourceMedia: MME_noNitrogen_noCarbon + Sodium L-Lactate (20 mM) + Ammonium chloride (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on 8-Mar-22
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 3 genes in this experiment
For carbon source Sodium L-Lactate in Pseudomonas fluorescens SBW25-INTG
For carbon source Sodium L-Lactate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Lactate utilization | 2 |
| L-rhamnose utilization | 1 |
| Murein hydrolase regulation and cell death | 1 |
| Respiratory dehydrogenases 1 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-lactaldehyde degradation (aerobic) | 2 | 2 | 1 |
| methylglyoxal degradation V | 3 | 2 | 1 |
| methylglyoxal degradation IV | 3 | 2 | 1 |
| superpathway of methylglyoxal degradation | 8 | 5 | 1 |
| lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) | 8 | 4 | 1 |
| superpathway of fucose and rhamnose degradation | 12 | 5 | 1 |