Experiment set1S93 for Rhodanobacter denitrificans MT42
R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Group: stressMedia: R2A_PIPES + Manganese (II) chloride tetrahydrate (1 mM), pH=7
Culturing: Rhodanobacter_MT42_ML2, 96 well deep well block, Aerobic, at 23 (C), shaken=700 rpm
By: Hans and Hira on 1/10/25
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate, 30 mM PIPES sesquisodium salt
Specific Phenotypes
For 8 genes in this experiment
For stress Manganese (II) chloride tetrahydrate in Rhodanobacter denitrificans MT42
For stress Manganese (II) chloride tetrahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Oxidative stress | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid biosynthesis
- Ubiquinone and menaquinone biosynthesis
- alpha-Linolenic acid metabolism
- Benzoate degradation via CoA ligation
- Limonene and pinene degradation
- Sulfur metabolism
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| assimilatory sulfate reduction III | 3 | 3 | 1 |
| assimilatory sulfate reduction I | 4 | 4 | 1 |
| superpathway of sulfate assimilation and cysteine biosynthesis | 9 | 8 | 1 |
| superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) | 10 | 8 | 1 |
| superpathway of L-methionine biosynthesis (by sulfhydrylation) | 12 | 11 | 1 |