Experiment set1S85 for Rhodanobacter denitrificans MT42

R2A_PIPES with Cadmium chloride 64 uM

Group: stress
Media: R2A_PIPES + Cadmium chloride (64 uM), pH=7
Culturing: Rhodanobacter_MT42_ML2, 96 well deep well block, Aerobic, at 23 (C), shaken=700 rpm
By: Hans and Hira on 1/10/25
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate, 30 mM PIPES sesquisodium salt

Specific Phenotypes

For 3 genes in this experiment

For stress Cadmium chloride in Rhodanobacter denitrificans MT42

For stress Cadmium chloride across organisms

SEED Subsystems

Subsystem	#Specific
Methionine Biosynthesis	1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	1
One-carbon metabolism by tetrahydropterines	1
Ribosomal protein S12p Asp methylthiotransferase	1
Serine-glyoxylate cycle	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Puromycin biosynthesis
• Lipopolysaccharide biosynthesis
• One carbon pool by folate
• Methane metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
folate transformations I	13	8	2
folate transformations III (E. coli)	9	9	1
reductive acetyl coenzyme A pathway I (homoacetogenic bacteria)	10	3	1
folate transformations II (plants)	11	10	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	25	1