Experiment set1S55 for Enterobacter sp. TBS_079

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L-Glutamine carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + L-Glutamine (20 mM)
Culturing: Enterobacter_TBS_079_ML3, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Robin on
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 5 genes in this experiment

For carbon source L-Glutamine in Enterobacter sp. TBS_079

For carbon source L-Glutamine across organisms

SEED Subsystems

Subsystem #Specific
Ammonia assimilation 1
Calvin-Benson cycle 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Methionine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamine degradation I 1 1 1
L-glutamate biosynthesis I 2 2 1
ammonia assimilation cycle III 3 3 1
S-adenosyl-L-methionine salvage II 3 2 1
S-adenosyl-L-methionine salvage I 4 4 1
L-asparagine biosynthesis III (tRNA-dependent) 4 3 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 3 1
L-methionine biosynthesis III 4 2 1
L-methionine biosynthesis I 5 5 1
seleno-amino acid biosynthesis (plants) 5 3 1
L-methionine biosynthesis II 6 5 1
L-glutamate and L-glutamine biosynthesis 7 5 1
superpathway of L-homoserine and L-methionine biosynthesis 8 8 1
L-citrulline biosynthesis 8 6 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 9 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 9 1
Rubisco shunt 10 9 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 10 1
superpathway of L-citrulline metabolism 12 8 1
Calvin-Benson-Bassham cycle 13 10 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
oxygenic photosynthesis 17 11 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 18 1
superpathway of seleno-compound metabolism 19 6 1
aspartate superpathway 25 24 1
ethene biosynthesis V (engineered) 25 18 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 19 1
1-butanol autotrophic biosynthesis (engineered) 27 19 1